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Type: Artigo de periódico
Title: An o-aminobenzoic acid film-based immunoelectrode for detection of the cardiac troponin T in human serum
Author: Mattos, AB
Freitas, TA
Kubota, LT
Dutra, RF
Abstract: An amperometric immunosensor for cardiac troponin T detection in human serum troponin T, a marker considered as "gold standard" for acute myocardial infarction diagnosis, is described. A stable carboxylic film to covalently bind antibodies against cTnT onto electrode surface was achieved with electropolymerization of the o-aminobenzoic acid. A fractional factorial study was performed to optimize the electropolymerization parameters. Cyclic voltammetry assays were carried out for characterize steps of the modified electrode surface. The obtained calibration curve at -0.05 V by amperometry presented a good linear response range from 0.05 to 5.0 ngmL(-1) cTnT with a correlation coefficient of 0.992 (n = 6) and 0.016 ng mL(-1) detection limit. The electrodes showed a good stability upon the analytical responses retaining 91.6% of its initial response after 18 days. This sensor showed outgoing results regarding sensitivity allowing reliable measurements of the cTnT at levels of clinical significance for acute myocardial infarctions diagnosis. (C) 2012 Elsevier B.V. All rights reserved.
Subject: Immunosensor
Poly(o-aminobenzoic acid)
Acute myocardial infarction
Cardiac troponin T
Country: Suíça
Editor: Elsevier Science Sa
Rights: fechado
Identifier DOI: 10.1016/j.bej.2012.12.005
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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