Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/544
Type: Artigo de periódico
Title: Infectivity of Strongyloides venezuelensis is influenced by variations in temperature and time of culture
Author: MACHADO, Eleuza Rodrigues
UETA, Marlene Tiduko
LOURENCO, Elaine Vicente
ANIBAL, Fernanda de Freitas
SILVA, Erica Vitalino Garcia da
ROQUE-BARREIRA, Maria Cristina
COSTA-CRUZ, Julia Maria
FACCIOLI, Lucia Helena
Abstract: The present research investigated the influence of temperature and time of larvae culture on the infectivity of Strongyloides venezuelensis. Mice were infected s.c. with 1500 larvae of S. venezuelensis maintained at 28 degrees C for three days of culture (dc), 28 degrees C for seven dc or 18 degrees C for seven dc. On days 1,3, 5, 7, 14 and 21 post-infection the animals were sacrificed and cell numbers in the blood, peritoneal cavity fluid (PCF), broncoalveolar fluid (BALF), cytokines, immunoglobulins, number of parasites and eggs/g of feces were quantified. Results demonstrated an increase in eosinophils and mononuclear cells in the blood, PCF and HALF of infected mice. Larvae at 28 degrees C/3dc induced earlier eosinophils in the PCF and HALF as opposed to larvae at 28 degrees C/7dc and 18 degrees C/7dc. Larvae at 28 degrees C/7dc induced higher synthesis of IL-4. IL-5 and IL-10 on days Sand 7 post-infection. Larvae at 28 degrees C/3dc in culture induced higher synthesis of IL-12 than larvae of seven dc, but time in culture induced better synthesis of IFN-gamma, after larval migration had ceased and only adult worms were present. Larvae at 28 degrees C/3dc in culture induced higher synthesis of IgG and IgG1 and expelled less female parasites than larvae cultivated for seven days. In conclusion, it was observed that the infectivity of S. venezuelensis is influenced by variations in temperature and time of culture. (C) 2010 Elsevier Inc. All rights reserved.
Subject: Strongyloides venezuelensis
Cytokine
Eosinophilia
Antibody
Mononuclear cells
Temperature
Time
Country: Estados Unidos
Editor: ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation: EXPERIMENTAL PARASITOLOGY, v.127, n.1, p.72-79, 2011
Rights: fechado
Identifier DOI: 10.1016/j.exppara.2010.06.027
Address: http://dx.doi.org/10.1016/j.exppara.2010.06.027
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Date Issue: 2011
Appears in Collections:IB - Artigos e Materiais de Revistas Científicas

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