Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/54095
Type: Artigo de periódico
Title: Acetylcholine receptor organization at the dystrophic extraocular muscle neuromuscular junction
Author: Marques, MJ
Pertille, A
Carvalho, CLT
Santo Neto, H
Abstract: Spared extraocular muscles of dystrophic mice are not subjected to regeneration process and can be used to verify whether the lack of dystrophin per se could cause changes in acetylcholine receptor (AChR) distribution. In the present study, rectus and oblique (spared) and retractor bulbi (nonspared) muscles were dissected from adult control (C57Bl/10) and mdx mice. AChRs and nerve terminals were labeled with rhodamine-a-bungarotoxin and anti-NF200-IgG-FITC, respectively, and visualized by confocal microscopy. Rectus and oblique muscles presented 0.5% central nucleation, while retractor bulbi had central nucleation in 45% of muscle fibers. In mdx rectus, AChRs were distributed in branches in 99% of the junctions examined (n = 200), similar to that observed for controls. Nerve terminals covered the AChR branches in 100% of the junctions examined. In control retractor bulbi, AChRs were distributed in regular branches. In mdx retractor bulbi, multiple fragmented islands of receptors were seen in 56% of the endplates examined (n = 200). These results suggest that the lack of dystrophin per se does not influence the distribution of acetylcholine receptors at the neuromuscular junction of spared extraocular muscles.
Subject: acetylcholine receptors
extraocular muscles
mdx mice
muscle regeneration
neuromuscular junction
Country: EUA
Editor: Wiley-liss
Rights: fechado
Identifier DOI: 10.1002/ar.20525
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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