Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/539
Type: Artigo de periódico
Title: Strongyloides venezuelensis: The antigenic identity of eight strains for the immunodiagnosis of human strongyloidiasis
Author: MACHADO, Eleuza Rodrigues
FACCIOLI, Lucia Helena
COSTA-CRUZ, Julia Maria
LOURENCO, Elaine Vicente
ROQUE-BARREIRA, Maria Cristina
PIRES, Maria do Rosario de Fatima Goncalves
UETA, Marlene Tiduko
Abstract: The antigens of eight strains of Strongyloides venezuelensis were identified by means of the indirect immunofluorescence antibody (IFAT), enzyme-linked immunosorbent assay (ELISA) and immunoblot (IB) tests. Infective larvae (L3) from these strains were obtained from Rattus norvegicus feces cultures. For IFAT, sections of U were used while the ELISA and IB, tests were conducted with alkaline extract. Ninety serum samples were tested: 30 from patients with S. stercoralis, 30 from patients with other parasitic diseases, and 30 from healthy subjects (free of parasites). Average sensitivity and specificity among all eight strains, both for IFAT and ELISA, were, respectively, 93% and 100%. In the IB, anti-S. stercoralis IgG recognized a single antigenic fraction with 45 kDa. Serum samples from patients with S. stercoralis revealed antigens from different strains of S. venezuelensis, indicating antigenic identity for possible use in the synthesis of recombinant antigen that could be useful in immunodiagnosis and vaccine against this parasite. (C) 2008 Elsevier Inc. All rights reserved.
Subject: Strongyloides venezuelensis
strongyloidiasis
diagnosis
antigen
antibodies
IFAT
ELISA
immunoblot
Country: Estados Unidos
Editor: ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation: EXPERIMENTAL PARASITOLOGY, v.119, n.1, p.7-14, 2008
Rights: fechado
Identifier DOI: 10.1016/j.exppara.2007.12.008
Address: http://dx.doi.org/10.1016/j.exppara.2007.12.008
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Date Issue: 2008
Appears in Collections:IB - Artigos e Materiais de Revistas Científicas

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