Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/53723
Type: Artigo de periódico
Title: A scheme for obtaining standards and HPLC quantification of leafy vegetable carotenoids
Author: Kimura, M
Rodriguez-Amaya, DB
Abstract: Reliability of data obtained by HPLC directly depends on the accuracy of the calibration. A major difficulty is obtaining and maintaining pure standards. This work presents a strategy for isolating standards by open column chromatography and quantification by HPLC, using leafy vegetables as examples. The purity of standards evaluated by HPLC was 91-97% for neoxanthin, 95-98% for violaxanthin, 97-100% for lactucaxanthin, 92-96% for lutein and 90-97% for beta-carotene. Calculation procedures were also evaluated, demonstrating that results obtained with one-point recalibration, straight-line equation (until 30 days after construction of the full calibration curves) and response factors relative to beta-carotene were similar (CVs of 1.6-4.0%), being well below between-sample lot natural variation (CVs of 6.1-42.5%). The scheme proposed is relatively low-cost, provides a constant supply of carotenoid standards, including those unavailable commercially, and high sample throughput. (C) 2002 Elsevier Science Ltd. All rights reserved.
Subject: carotenoids
HPLC
analysis
standards
Country: Inglaterra
Editor: Elsevier Sci Ltd
Rights: fechado
Identifier DOI: 10.1016/S0308-8146(02)00203-0
Date Issue: 2002
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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