Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/53717
Type: Artigo de periódico
Title: A ROOM-TEMPERATURE, RAPID METHOD FOR THE DETERMINATION OF PROTEIN IN WHEAT AND OTHER GRAINS BY THE BIURET REACTION
Author: STRONG, FC
DUARTE, AMA
Abstract: A rapid, room-temperature, biuret method for determining protein in grains was developed. Samples of wheat ground to pass a 0.105-mm screen were mixed for 5 min in a blender with 2 ml of isopropanol, 700 mg of basic cupric carbonate (bright green), and 100 ml of 0.500 M NaOH in 30% isopropanol. After filtering with suction through a glass fiber filter, absorbance was measured at 550 nm. A calibration curve for wheat of absorbance vs. protein concentration gave a slope of 0.350 cm-1 g-1 L, compared to 0.3 10 for the official AACC method using heat. Satisfactory results were also obtained with rice and soybeans.
Editor: Amer Assoc Cereal Chemists
Rights: fechado
Date Issue: 1992
Appears in Collections:Unicamp - Artigos e Outros Documentos

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