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Type: Artigo de periódico
Title: A reagentless amperometric carbon paste based sensor for NADH
Author: Malinauskas, A
Ruzgas, T
Gorton, L
Kubota, LT
Abstract: Carbon paste electrodes, based on zirconium phosphate with immobilized N-methylphenazonium or Nile blue, were prepared. The electrodes show anodic current response to NADH at an operating potential ranging from - 0.05 to 0.30 V (vs. SCE). The sensitivity of electrodes to NADH was shown to increase with increasing operation potential. It was shown that the concentration dependences for electrodes, obtained at different operating potentials. follow Michaelis-Menten scheme. confirming an electrodcatalytic mechanism for electrooxidation of NADH with a precursor complex formation. The detection limit for NADH was found to be ca. 2 mu M for both types of sensors.
Subject: biosensor
carbon paste electrode
Country: Alemanha
Editor: Wiley-v C H Verlag Gmbh
Citation: Electroanalysis. Wiley-v C H Verlag Gmbh, v. 12, n. 3, n. 194, n. 198, 2000.
Rights: fechado
Identifier DOI: 10.1002/(SICI)1521-4109(200002)12:3<194
Date Issue: 2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

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