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Type: Artigo de periódico
Title: A pseudobiospecific hollow fiber cartridge for in vitro adsorption of autoantibodies from pathological serum
Author: Ventura, RCA
Zollner, RL
Legallais, C
Vijayalakshmi, MA
Bueno, SMA
Abstract: The affinity filtration technique using histidine as a pseudobiospecific ligand immobilized on poly(ethylene vinyl alcohol) hollow fiber membranes (His-PEVA) was used to remove autoantibodies from serum of patients with autoimmune disease. The effects of buffering solution conditions on the efficiency of autoantibodies removal was studied. The removal of anti-dsDNA, anti-SS-A/Ro, anti-Sm, anti-Sm/RNP and anti-cardiolipin autoantibodies present in the serum was investigated, comparing the efficiency between Hepes and Tris-HCl buffers. The results showed the potential of the membrane to remove all the autoantibodies studied. Anti SS-A/Ro was removed more efficiently in Tris-HCl buffer system rather than with the Hepes buffer.
Subject: autoantibodies
auto-immune diseases
extracorporeal circulation
affinity membranes
Country: Brasil
Editor: Brazilian Soc Chemical Eng
Rights: aberto
Date Issue: 2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

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