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Type: Artigo de periódico
Title: Metabolic Alterations in Different Developmental Stages of Pilocarpus microphyllus
Author: Abreu, IN
Choi, YH
Sawaya, ACHF
Eberlin, MN
Mazzafera, P
Verpoorte, R
Abstract: Pilocarpine is an imidazole alkaloid that has been used for more than a century in glaucoma treatment. It is present in several species of the Pilocarpus genus (jaborandi), with its highest concentrations in P. microphyllus. In addition to pilocarpine, pilosine - an imidazole alkaloid without pharmacological use - is produced in high concentrations in mature plants. A metabolomic study was carried out on juvenile and mature plants to obtain information about pilocarpine metabolism at different developmental stages. Methanol-water and alkaloid extracts were analyzed by (1)H NMR and ESI-MS. Metabolic profiles from both techniques showed clear differences between various developmental stages. Intense signals in the aromatic region of the (1)H NMR spectrum and ions from pilosine and related alkaloids by ESI/MS were found only in extracts from mature plant. Two new imidazole alkaloids were identified by MS(n). Our results suggest that pilosine is produced exclusively in mature developmental stage, and juvenile plant material seems to be appropriate for further studies on pilocarpine biosynthesis.
Subject: jaborandi
Pilocarpus microphyllus
metabolic change
Country: Alemanha
Editor: Georg Thieme Verlag Kg
Rights: aberto
Identifier DOI: 10.1055/s-0030-1250314
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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