Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/53565
Type: Artigo de periódico
Title: Meiosis aspects and nucleolar activity in Triatoma vitticeps (Triatominae, Heteroptera)
Author: Severi-Aguiar, GDC
Lourenco, LB
Bicudo, HEMC
Azeredo-Oliveira, MTV
Abstract: Some aspects of both the nucleolar organizer activity and meiosis were studied in the testes of Triatoma vitticeps (Heteroptera, Triatominae). The techniques used included squashing followed by lacto-acetic orcein staining, silver-ion impregnation, fluorescent banding (CMA(3), Quinacrine mustard and DAPI) and fluorescent in situ hybridization (FISH). A close relationship between heterochromatin and nucleolus in testicular cells was observed. During meiosis, the silver-ion impregnation pattern varied. At metaphase plate, a small body appeared apart from the chromosomes. In the spermatids this small body was seen in preparations stained with orcein and silver- ion impregnation but not with fluorochromes or FISH. These characteristics combined suggest that these corpuscles represent a source of ribonucleoproteins (RNP) - RNA and specific nucleolar proteins. Silver-ion impregnation and (FISH) revealed nucleolar organizer activity in two metaphase sex chromosomes (X). These results indicate that, in these species, nucleolar organizer regions (NORs) are located in the sex chromosomes, X chromosomes were were CMA(3)(+) and Y chromosome was DAPI(+).
Subject: fluorescent in situ hybridization
meiosis
nucleolar organizer activity
ribonucleoproteins (RNP)
silver impregnation
Country: Holanda
Editor: Springer
Rights: fechado
Identifier DOI: 10.1007/s10709-005-1443-2
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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