Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/53457
Type: Artigo de periódico
Title: Measuring electrical and mechanical properties of red blood cells with double optical tweezers
Author: Fontes, A
Fernandes, HP
De Thomaz, AA
Barbosa, LC
Barjas-Castro, ML
Cesar, CL
Abstract: Red blood cell (RBC) aggregation in the blood stream is prevented by the zeta potential created by its negatively charged membrane. There are techniques, however, to decrease the zeta potential and allow cell agglutination, which are the basis of most of antigen-antibody tests used in immunohematology. We propose the use of optical tweezers to measure membrane viscosity, adhesion, zeta potential, and the double layer thickness of charges (DLT) formed around the cell in an electrolytic solution. For the membrane viscosity experiment, we trap a bead attached to RBCs and measure the force to slide one RBC over the other as a function of the velocity. Adhesion is quantified by displacing two RBCs apart until disagglutination. The DLT is measured using the force on the bead attached to a single RBC in response to an applied voltage. The zeta potential is obtained by measuring the terminal velocity after releasing the RBC from the trap at the last applied voltage. We believe that the methodology proposed here can provide information about agglutination, help to improve the tests usually performed in transfusion services, and be applied for zeta potential measurements in other samples. (C) 2008 Society of Photo-Optical Instrumentation Engineers.
Subject: optical tweezers
red blood cell
agglutination
membrane viscosity
adhesion
zeta potential
Country: EUA
Editor: Spie-soc Photoptical Instrumentation Engineers
Rights: aberto
Identifier DOI: 10.1117/1.2870108
Date Issue: 2008
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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