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Type: Artigo de periódico
Title: Matrix metalloproteinase-2 may be involved with increased bone loss associated with experimental periodontitis and smoking: A study in rats
Author: Neto, JBC
de Souza, AP
Barbieri, D
Moreno, H
Sallum, EA
Nociti, FH
Abstract: Background: Smoking has been associated with periodontitis severity and is considered a risk factor for its development. It has been reported that matrix metalloproteinase (MMP) produced by host cells plays a major role in periodontal tissue destruction. Thus, the present study tested, in rats, the hypothesis that local increased levels of MMP-2 would be associated with the enhanced periodontitis-related bone loss after intermittent cigarette smoke inhalation (CSI). Methods: Twenty-seven adult male Wistar rats were used. A ligature was placed around one of the mandibular first molars of each animal and they were randomly assigned to the following control (N = 13) or CSI (N = 14) group. Sixty days later, the animals were sacrificed, the gingival tissues harvested, and the specimens processed for decalcified sections. Extracts from the gingival tissues were prepared and assayed for MMP-2 expression. Results: Intergroup comparisons (unligated sites) showed that CSI might directly affect alveolar bone (0.16 +/- 0.03 mm(2) versus 0.24 +/- 0.09 mm(2) for non-smokers and smokers, respectively; P = 0.001). Moreover, CSI significantly enhanced bone loss resulting from experimental periodontitis (0.64 +/- 0.36 mm(2) versus 1.50 +/- 0.50 mm(2) for non-smokers and smokers, respectively; P <0.05). In addition, zymography demonstrated that CSI also enhanced both MMP-2 levels and activity in the gingival tissues around ligated teeth. Conclusion: Within the limits of the present investigation, it can be assumed that the effect of CSI on MMP-2 levels and activity may account for the increased periodontitis progression rate observed in smokers.
Subject: animal studies
bone loss
risk factors
smoking/adverse effects
Country: EUA
Editor: Amer Acad Periodontology
Rights: fechado
Identifier DOI: 10.1902/jop.2004.75.7.995
Date Issue: 2004
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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