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|Type:||Artigo de periódico|
|Title:||Mapping QTLs for kernel oil content in a tropical maize population|
de Souza, CL
de Souza, AP
|Abstract:||Maize cultivars often have low kernel oil content. To increase the oil content, efficient maize breeding programs have to be developed, which require the knowledge of the inheritance of this trait. Thus, the objective of this research was to map quantitative trait locus (QTLs) and estimate their effects for kernel oil content in a tropical maize population. Two maize inbred lines, contrasting for kernel oil content, were used to develop an F-2 population. Four hundred and eight F-2 plants were self-pollinated, and their kernels (F-2:3 progenies) were used for kernel oil evaluation. A genetic map with 75 microsatellites was developed, and the QTLs were mapped using the composite interval map (CIM); also, estimates of genetic and phenotypic variances, and heritability coefficient were computed. The map presented 10 linkage groups, spanned 1,438.6 cM in length with an average interval of 19.18 cM between adjacent markers. The kernel oil content averaged 58.40 g kg(-1), and the broad-sense heritability was high (h(2)=0.98). Thirteen QTLs were mapped, which were distributed into eight chromosomes, and explained 26.64% of the genetic variation. QTLs in chromosomes 1, 5, and 6 contributed the most for kernel oil content. Nine out of 13 QTLs with favorable alleles were from the parental inbred with the highest kernel oil content. The average level of dominance was partial, but gene action of the QTLs ranged from additive to overdominance. Eight out of 13 mapped QTLs were already reported for temperate maize populations.|
|Subject:||composite interval mapping|
quantitative trait loci
tropical maize germplasm
Zea mays L.
|Editor:||Kluwer Academic Publ|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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