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Type: Artigo de periódico
Title: Low temperature and aging-promoted expression of PUMP in potato tuber mitochondria
Author: Nantes, IL
Fagian, MM
Catisti, R
Arruda, P
Maia, IG
Vercesi, AE
Abstract: In this communication, we show that the plant uncoupling mitochondrial protein (PUMP) present in potato tuber mitochondria is induced by aging at 28 degrees C and that this induction is strongly stimulated when the potato tubers are stored at low temperature (4 degrees C). PUMP activity was detected by the degree of linoleic acid (LA)-induced ATP-sensitive mitochondrial uncoupling measured as a function of the decrease in membrane potential(Delta Psi). The PUMP content was evaluated by immunoblot analysis using polyclonal antibodies raised against potato PUMP that specifically detected a 32 kDa band. In agreement with the effect of LA on AY, the content of the 32 kDa band increased during storage and was stimulated by low temperature. These results support the proposed role of PUMP in plant thermogenesis and possibly in fruit ripening and senescence. (C) 1999 Federation of European Biochemical Societies.
Subject: plant uncoupling mitochondrial protein
potato tuber mitochondrion
reactive oxygen
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Identifier DOI: 10.1016/S0014-5793(99)01017-0
Date Issue: 1999
Appears in Collections:Unicamp - Artigos e Outros Documentos

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