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Type: Artigo de periódico
Title: Low protein diet confers resistance to the inhibitory effects of interleukin1 beta on insulin secretion in pancreatic islets
Author: Vieira, EC
Carneiro, EM
Latorraca, MQ
Delguingaro-Augusto, V
Amaral, MEC
Bosqueiro, JR
Boschero, AC
Abstract: High protein content in the diet during childhood and adolescence has been associated to the onset insulin-dependent diabetes mellitus. We investigated the effect of interleukin-1 beta (IL-I beta) on insulin secretion, glucose metabolism, and nitrite formation by islets isolated from rats fed with normal protein (NP, 17%) or low protein (LP, 6%) after weaning. Pretreatment of islets with IL-1 beta for 1 h or 34 h inhibited the insulin secretion induced by glucose in both groups, but it was less marked in LP than in NP group. Islets from LP rats exhibited a decreased IL-1 beta -induced nitric oxide (NO) production, lower inhibition of D-[(UC)-C-14]-glucose oxidation to (CO2)-C-14, and less pronounced effect of IL-1 beta on alpha -ketoisocaproic acid-induced insulin secretion than NP islets. However, when the islets were stimulated by high concentrations of K+ the inhibitory effect of IL-1 beta on insulin secretion was not different between groups. In conclusion, protein restriction protects beta -cells of the deleterious effect of IL-1 beta, apparently, by decreasing NO production. The lower NO generation in islets from protein deprived rats may be due to increased free fatty acids oxidation and consequent alteration in Ca2+ homeostasis. (C) 2001 Elsevier Science Inc. All rights reserved.
Subject: protein malnutrition
pancreatic islets
insulin secretion
interleukin 1 beta
beta cell
Country: EUA
Editor: Elsevier Science Inc
Citation: Journal Of Nutritional Biochemistry. Elsevier Science Inc, v. 12, n. 5, n. 285, n. 291, 2001.
Rights: fechado
Identifier DOI: 10.1016/S0955-2863(01)00140-1
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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