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Type: Artigo de periódico
Title: Investigation of charged polymer influence on green fluorescent protein thermal stability
Author: NOVAES, Leticia Celia de Lencastre
MAZZOLA, Priscila Gava
PESSOA JR., Adalberto
PENNA, Thereza Christina Vessoni
Abstract: Methods of stabilization and formulation of proteins are important in both biopharmaceutical and biocatalysis industries. Polymers are often used as modifiers of characteristics of biological macromolecules to improve the biochemical activity and stability of proteins or drug bioavailability. Green fluorescent protein (GFP) shows remarkable structural stability and high fluorescence; its stability can be directly related to its fluorescence output, among other characteristics. GFP is stable under increasing temperatures, and its thermal denaturation is highly reproducible. Relative thermal stability was undertaken by incubation of GFP at varying temperatures and GFP fluorescence was used as a reporter for unfolding. At 80 degrees C, DEAE-dextran did not have any effect on GFP fluorescence, indicating that it does not confer stability.
Country: Holanda
Citation: NEW BIOTECHNOLOGY, v.28, n.4, p.391-395, 2011
Rights: fechado
Date Issue: 2011
Appears in Collections:FCM - Artigos e Outros Documentos

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