Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/444
Type: Artigo
Title: Age-related association of rDNA and telomeres with the nuclear matrix in mouse hepatocytes
Author: Moraes, Alberto S.
Mondin, Mateus
Beletti, Marcelo E.
Aguiar-Perecin, Margarida L. R.
Guaraldo, Ana M. A.
Mello, Maria Luiza S.
Abstract: Transcribed sequences have been suggested to be associated with the nuclear matrix, differing from non-transcribing sequences, which have been reported to be contained in DNA loops. However, although a dozen of genes have their expression level affected by aging, data on chromatin-nuclear matrix interactions under this physiological condition are still scarce. In the present study, liver imprints from young, adult and old mice were subjected to FISH (fluorescence in situ hybridization) for 45S rDNA and telomeric sequences, with or without a lysis treatment to produce extended chromatin fibres. There was an increased amount of 45S rDNA sequences located in DNA loops as the animals grow older, while telomeric sequences were always observed in DNA loops irrespective of the animal age. We assume that active rRNA genes associate with the nuclear matrix, while DNA loops contain silent sequences. Transcription of each 45S rDNA repeat unit is suggested to be dependent on its interaction with the nuclear matrix.
Transcribed sequences have been suggested to be associated with the nuclear matrix, differing from non-transcribing sequences, which have been reported to be contained in DNA loops. However, although a dozen of genes have their expression level affected by aging, data on chromatin-nuclear matrix interactions under this physiological condition are still scarce. In the present study, liver imprints from young, adult and old mice were subjected to FISH (fluorescence in situ hybridization) for 45S rDNA and telomeric sequences, with or without a lysis treatment to produce extended chromatin fibres. There was an increased amount of 45S rDNA sequences located in DNA loops as the animals grow older, while telomeric sequences were always observed in DNA loops irrespective of the animal age. We assume that active rRNA genes associate with the nuclear matrix, while DNA loops contain silent sequences. Transcription of each 45S rDNA repeat unit is suggested to be dependent on its interaction with the nuclear matrix.
Subject: Envelhecimento
Cromatina
Matriz nuclear
DNA ribossômico
Telômeros
Hepatócitos
Country: Inglaterra
Editor: Academic Press
Citation: CELL BIOLOGY INTERNATIONAL, v.34, n.9, p.925-931, 2010
Rights: aberto
Identifier DOI: 10.1042/CBI20090457
Address: https://onlinelibrary.wiley.com/doi/abs/10.1042/CBI20090457
Date Issue: 2010
Appears in Collections:IB - Artigos e Outros Documentos

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