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Type: Artigo de periódico
Title: Production and characterization of surface-active compounds from Gordonia amicalis
Author: Jackisch-Matsuura, Ani Beatriz
Santos, Leonardo Silva
Eberlin, Marcos Nogueira
Faria, Andréia Fonseca de
Matsuura, Takeshi
Grossman, Matthew James
Durrant, Lucia Regina
Abstract: Two methods were used to make crude preparations of surface-active compounds (SACs) produced by Gordonia amicalis grown on the medium containing 1% diesel oil. Using a 2:1 (v/v) solution of chloroform:methanol for extraction, Type I SACs were isolated and shown to produce oil in water (O/W) emulsions. Type II SACs were isolated by precipitation with ammonium sulfate and produced predominantly water in oil emulsions (W/O). The crude Type I and II preparations were able to produce a significant reduction in the surface tension of water; however, the crude Type II preparation had 10-25 fold higher emulsification activity than the Type I preparation. Both SAC preparations were analyzed by the TLC and each produced two distinct bands with Rf 0.44 and 0.62 and Rf 0.52 and 0.62, respectively. The partially purified SACs were characterized by the ESI(+)-MS, FT-IR and NMR. In each one of these fractions, a mixture of 10 oligomers was found consisting of a series of compounds, with masses from 502 to 899, differing in molecular mass by a repeating unit of 44 Daltons. The mass spectra of these compounds did not appear to match other known biosurfactants and could represent a novel class of these compounds.
Subject: Bioemulsifier
Gordonia amicalis
industrial chemicals
microbial surfactant production
Editor: Instituto de Tecnologia do Paraná - Tecpar
Rights: aberto
Identifier DOI: 10.1590/S1516-89132014000100019
Date Issue: 1-Feb-2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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