Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/428
Type: Artigo de periódico
Title: Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis
Author: OLDONI, Tatiane Luiza C.
CABRAL, Ingridy S. R.
D`ARCE, Marisa A. B. Regitano
ROSALEN, Pedro L.
IKEGAKI, Masaharu
NASCIMENTO, Andrea M.
ALENCAR, Severino M.
Abstract: Activity-directed fractionation and purification processes were employed to identify isoflavonoids with antioxidant and antimicrobial activities from Brazilian red propolis. Crude propolis was extracted with ethanol (80%. v/v) and fractioned by liquid-liquid extraction technique using hexane and chloroform. Since chloroform fraction showed strong antioxidant and antimicrobial activities it was purified and isolated using various chromatographic techniques. Comparing our spectral data (UV, NMR, and mass spectrometry) with values found in the literature, we identified two bioactive isoflavonoids (vestitol and neovestitol), together with one chalcone (isoliquiritigenin). Vestitol presented higher antioxidant activity against beta-carotene consumption than neovestitol. The antimicrobial activity of these three compounds against Staphylococcus aureus, Streptococcus mutans, and Actinomyces naeslundii was evaluated and we concluded that isoliquiritigenin was the most active one with lower MIC, ranging from 15.6 to 62.5 mu g/mL. Our results showed that Brazilian red propolis has biologically active isoflavonoids that may be used as a mild antioxidant and antimicrobial for food preservation. (C) 2010 Elsevier B.V. All rights reserved.
Subject: Isoflavonoids
Chalcone
Antioxidant activity
Antimicrobial activity
Brazilian propolis
Country: Holanda
Editor: ELSEVIER SCIENCE BV
Rights: fechado
Identifier DOI: 10.1016/j.seppur.2010.12.007
Address: http://apps.isiknowledge.com/InboundService.do?Func=Frame&product=WOS&action=retrieve&SrcApp=EndNote&UT=000287988500004&Init=Yes&SrcAuth=ResearchSoft&mode=FullRecord
http://dx.doi.org/10.1016/j.seppur.2010.12.007
Date Issue: 2011
Appears in Collections:FOP - Artigos e Outros Documentos

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