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Type: Artigo de periódico
Title: Precipitation of Porcine Insulin With Carbon Dioxide
Author: TASHIMA, Alexandre Keiji
OTTENS, Marcel
WIELEN, Luuk A. M. van der
CINTRA, Dennys E.
PAULI, Jose R.
PESSOA FILHO, Pedro de Alcantara
MIRANDA, Everson Alves
Abstract: Recent works have pointed to the use of volatile electrolytes such as carbon dioxide (CO(2)) dissolved in aqueous solutions as a promising alternative to the precipitating agents conventionally used for protein recovery in the food and pharmaceutical industries. In this work we investigated experimental and theoretical aspects of the precipitation of porcine insulin, a biomolecule of pharmaceutical interest, using CO(2) as an acid- precipitating agent. The Solubility of porcine insulin in NaHCO(3) solutions in pressurized CO(2) was determined as a function of temperature and pressure, with a minimum being observed close to the protein isoclectric point. A thermodynamic model was developed and successfully utilized to correlate the experimental data. Insulin was considered a polyelectrolyte in the model and its self-association reactions were also taken into account. The biological activity of insulin was maintained after precipitation With CO(2), although some activity can be lost if foam is formed in the depressurization step. Biotechnol. Bioeng. 2009;103: 909-919. (C) 2009 Wiley Periodicals, Inc.
Subject: downstream
carbon dioxide
Country: Estados Unidos
Rights: fechado
Date Issue: 2009
Appears in Collections:FEQ - Artigos e Outros Documentos

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