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Type: Artigo de periódico
Title: Neuromuscular activity of Bothrops neuwiedi pauloensis snake venom in mouse nerve-muscle preparations
Author: Durigon, A. M.
Borja-Oliveira, C. R.
Dal Belo, C. A.
Oshima-Franco, Y.
Cogo, J. C.
Lapa, A. J.
Souccar, C.
Rodrigues-Simioni, L.
Abstract: The pharmacological effects of Bothrops neuwiedi pauloensis venom on mouse phrenic nerve-diaphragm (PND) preparations were studied. Venom (20 mug/ml) irreversibly inhibited indirectly evoked twitches in PND preparations (60 &plusmn; 10% inhibition, mean &plusmn; SEM; p<0.05; n=6). At 50 mug/ml, the venom blocked indirectly and directly (curarized preparations) evoked twitches in mouse hemidiaphragms. In the absence of Ca2+, venom (50 mug/ml), produced partial blockade only after an 80 min incubation, which reached 40.3 &plusmn; 7.8% (p<0.05; n=3) after 120 min. Venom (20 mug/ml) increased (25 &plusmn; 2%, p< 0.05) the frequency of giant miniature end-plate potentials in 9 of 10 end-plates after 30 min and the number of miniature end-plate potentials which was maximum (562 &plusmn; 3%, p<0.05) after 120 min. During the same period, the resting membrane potential decreased from - 81 &plusmn; 1.4 mV to - 41.3 &plusmn; 3.6 mV 24 fibers; p<0.01; n=4) in the end-plate region and from - 77.4 &plusmn; 1.4 to -44.6 &plusmn; 3.9 mV (24 fibers; p<0.01; n=4) in regions distant from the end-plate. These results indicate that B. n. pauloensis venom acts primarily at presynaptic sites. They also suggest that enzymatic activity may be involved in this pharmacological action.
Subject: giant MEPPs
presynaptic action
Editor: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
Rights: aberto
Identifier DOI: 10.1590/S1678-91992005000100004
Date Issue: 1-Mar-2005
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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