Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/37954
Type: Artigo de periódico
Title: Identification of SNPs in RNA-seq data of two cultivars of Glycine max (soybean) differing in drought resistance
Author: Vidal, Ramon Oliveira
Nascimento, Leandro Costa do
Maurício Costa Mondego, Jorge
Amarante Guimarães Pereira, Gonçalo
Falsarella Carazzolle, Marcelo
Abstract: The legume Glycine max (soybean) plays an important economic role in the international commodities market, with a world production of almost 260 million tons for the 2009/2010 harvest. The increase in drought events in the last decade has caused production losses in recent harvests. This fact compels us to understand the drought tolerance mechanisms in soybean, taking into account its variability among commercial and developing cultivars. In order to identify single nucleotide polymorphisms (SNPs) in genes up-regulated during drought stress, we evaluated suppression subtractive libraries (SSH) from two contrasting cultivars upon water deprivation: sensitive (BR 16) and tolerant (Embrapa 48). A total of 2,222 soybean genes were up-regulated in both cultivars. Our method identified more than 6,000 SNPs in tolerant and sensitive Brazilian cultivars in those drought stress related genes. Among these SNPs, 165 (in 127 genes) are positioned at soybean chromosome ends, including transcription factors (MYB, WRKY) related to tolerance to abiotic stress.
Subject: single nucleotide polymorphisms
deep sequencing
drought resistance
Editor: Sociedade Brasileira de Genética
Rights: aberto
Identifier DOI: 10.1590/S1415-47572012000200014
Address: http://dx.doi.org/10.1590/S1415-47572012000200014
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200014
Date Issue: 1-Jan-2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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