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Type: Artigo de periódico
Title: Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit
Author: Rodrigues, Fabiana Aparecida
Marcolino-Gomes, Juliana
Carvalho, Josirlei de Fátima Corrêa
Nascimento, Leandro Costa do
Neumaier, Norman
Farias, José Renato Bouças
Carazzolle, Marcelo Falsarella
Marcelino, Francismar Corrêa
Nepomuceno, Alexandre Lima
Abstract: Soybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes expressed during the early response to water deficit in roots and leaves. These genes were compared among the lines to assess probable differences in the plant transcriptomes. In general, similar biochemical processes were predominant in both cultivars; however, there were more considerable differences between roots and leaves of Embrapa 48. Moreover, we present here a fast, clean and straightforward method to obtain drought-stressed root tissues and a large enriched collection of transcripts expressed by soybean plants under water deficit that can be useful for further studies towards the understanding of plant responses to stress.
Subject: gene expression
Editor: Sociedade Brasileira de Genética
Rights: aberto
Identifier DOI: 10.1590/S1415-47572012000200011
Date Issue: 1-Jan-2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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