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Type: Artigo
Title: Inhibition of S. mutans after nanoparticle mediated photodynamic antimicrobial chemotherapy on oral biofilm flow-cell system using laser or LED
Author: Moreira, Kelly Maria Silva
Graziano, Talita S.
Sales, Luciana Solera
Joia, Felipe
Stipp, Rafael Nobrega
Puppin-Rontani, Regina Maria
Nobre-dos-Santos, Marinês
Rodrigues, Lidiany Karla Azevedo
Steiner-Oliveira, Carolina
Abstract: This study tested the effect of photodynamic antimicrobial chemotherapy (PACT) on single-species biofilm grown in a flow-cell system using methylene blue associated with nanoparticles of β-cyclodextrin and light sources of red laser or light-emitting diode (LED). The flow-cell system comprised chambers for biofilm cultivation with S. mutans under continuous hydrodynamic conditions containing BHI supplemented with 1% sucrose (w/v). Biofilms were divided into six groups (n = 6): C (control), P (photosensitizer + β-cyclodextrin), L (laser), LED (light-emitting diode), LP (laser + photosensitizer + β-cyclodextrin), and LEDP (LED + photosensitizer + β-cyclodextrin). Groups irradiated with laser were exposed to a low power light (λ = 660 nm, 320 J/cm2, 0.1 W, 9 J, 90 s). Groups irradiated with LEDs were exposed to 12 LEDs (λ = 660 nm, 8.1 J/cm2, 0.09 W/cm2, and 8.1 J for 90 s). The antibacterial potential of treatments was assessed by viable S. mutans counts of biofilm in selective medium. The vitality of intact biofilms was qualitatively evaluated by confocal laser scanning microscopy analysis (CLSM). The data were analyzed by Welch’s one-way ANOVA followed by Scheffe´s post-hoc tests (p < 0.05). No reductions in the number of S. mutans for C, P, L, and LED groups were found (p > 0.05). The number of S. mutans was significantly reduced in LP and LEDP groups (p < 0.05) and LP had the lowest microbial numbers (p < 0.05). PACT protocol was effective in reducing S. mutans in the dynamic biofilm model tested using laser or LED. Furthermore, laser as a light source presented a better performance than LED
Subject: Biofilmes
Country: Alemanha
Editor: Springer
Rights: Fechado
Identifier DOI: 10.1007/s41547-021-00128-9
Date Issue: 2021
Appears in Collections:FOP - Artigos e Outros Documentos

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