Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/364089
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampAtaide, Janaína Artem-
dc.contributor.authorunicampGeraldes, Danilo Costa-
dc.contributor.authorunicampGérios, Eloah Favero-
dc.contributor.authorunicampNascimento, Laura de Oliveira-
dc.contributor.authorunicampMazzola, Priscila Gava-
dc.typeArtigopt_BR
dc.titleFreeze-dried chitosan nanoparticles to stabilize and deliver bromelainpt_BR
dc.contributor.authorAtaide, Janaína Artem-
dc.contributor.authorGeraldes, Danilo Costa-
dc.contributor.authorGérios, Eloah Favero-
dc.contributor.authorBissaco, Fernanda Mazon-
dc.contributor.authorCefali, Letícia Caramori-
dc.contributor.authorOliveira-Nascimento, Laura-
dc.contributor.authorMazzola, Priscila Gava-
dc.subjectBromelinapt_BR
dc.subject.otherlanguageBromelinpt_BR
dc.description.abstractBromelain has many therapeutic properties including wound healing and blood circulation improvement. However, bromelain is usually unstable and suffers autolysis, which results in a decrease of enzymatic activity and limited applications. Encapsulation of bromelain in nanoparticles can diminish degradation by protease immobilization, which may increase its stability and efficacy. The natural polymer chitosan can be nanostructured and trap the enzyme, maintaining the claims of biocompatibility, biodegradability and natural source of the formulation ingredients. Considering the above, chitosan-bromelain nanoparticles (C-B-NP) were produced by ionic crosslinking and presented spherical shape (electron microscopy) of 100.9 ± 0.5 nm and polydispersity index of 0.222 ± 0.012 (dynamic light scattering). Encapsulation efficiency was calculated as 85.1 ± 1% with regard to enzymatic activity, and C-B-NP presented 4.9 U/mL of enzymatic activity, which corresponds to 104.7% of free bromelain activity, confirming its integrity upon encapsulation. However, C-B-NP were unstable when stored in aqueous suspension, leading to freeze-drying studies of the formulation. Glycine and maltose were used as potential lyoprotectors and the formulation optimized by a factorial design; resultant products presented short resuspension time, slightly altered nanoparticles mean size and increased encapsulation rate compared to the previous liquid form. Maltose was able to better maintain formulation's enzymatic activity over time (90 days), especially when stored under refrigeration. Therefore, freeze-dried C-B-NP can effectively improve bromelain and nanoparticle stability, which allow further in vivo applications as a dried powder for topical administration or as a raw material for other dosage formspt_BR
dc.relation.ispartofJournal of drug delivery science and technologypt_BR
dc.publisher.cityAmsterdampt_BR
dc.publisher.countryPaíses Baixospt_BR
dc.publisherElsevierpt_BR
dc.date.issued2021-
dc.date.monthofcirculationFeb.pt_BR
dc.language.isoengpt_BR
dc.description.volume61pt_BR
dc.rightsFechadopt_BR
dc.identifier.issn1773-2247pt_BR
dc.identifier.eissn2588-8943pt_BR
dc.identifier.doi10.1016/j.jddst.2020.102225pt_BR
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S1773224720315148pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber404229/2016–6; 301436/2017-7pt_BR
dc.description.sponsordocumentnumber2015/15068–5; 2016/02282–1; 2016/02287–3; 2016/03444–5pt_BR
dc.date.available2021-08-31T17:27:09Z-
dc.date.accessioned2021-08-31T17:27:09Z-
dc.description.provenanceSubmitted by Mariana Aparecida Azevedo (mary1@unicamp.br) on 2021-08-31T17:27:09Z No. of bitstreams: 1 E3927A9E-ACBF.pdf: 6307139 bytes, checksum: bb1380c73b7aec2e245a19361f0ef196 (MD5)en
dc.description.provenanceMade available in DSpace on 2021-08-31T17:27:09Z (GMT). No. of bitstreams: 1 E3927A9E-ACBF.pdf: 6307139 bytes, checksum: bb1380c73b7aec2e245a19361f0ef196 (MD5) Previous issue date: 2021en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/364089-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.unidadeFaculdade de Ciências Farmacêuticaspt_BR
dc.contributor.unidadeFaculdade de Ciências Farmacêuticaspt_BR
dc.contributor.unidadeFaculdade de Ciências Farmacêuticaspt_BR
dc.contributor.unidadeFaculdade de Ciências Farmacêuticaspt_BR
dc.contributor.unidadeFaculdade de Ciências Farmacêuticaspt_BR
dc.subject.keywordNanoencapsulationpt_BR
dc.subject.keywordFormulation stabilitypt_BR
dc.subject.keywordFreeze-dryingpt_BR
dc.identifier.sourceE3927A9E-ACBFpt_BR
dc.creator.orcid0000-0002-1929-9474pt_BR
dc.creator.orcid0000-0002-7091-2895pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0002-2831-4795pt_BR
dc.creator.orcid0000-0002-3795-8189pt_BR
dc.type.formArtigo de pesquisapt_BR
dc.identifier.articleid102225pt_BR
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