Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/364055
Full metadata record
DC FieldValueLanguage
dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampClerici, Stefano Piatto-
dc.contributor.authorunicampConsonni, Sílvio Roberto-
dc.contributor.authorunicampFerreira, Carmen Veríssima-
dc.typeArtigopt_BR
dc.titleColorectal cancer cell-derived small extracellular vesicles educate human fibroblasts to stimulate migratory capacitypt_BR
dc.contributor.authorClerici, Stefano Piatto-
dc.contributor.authorPeppelenbosch, Maikel-
dc.contributor.authorFuhler, Gwenny-
dc.contributor.authorConsonni, Sílvio Roberto-
dc.contributor.authorFerreira-Halder, Carmen Veríssima-
dc.subjectFibroblastos associados a câncerpt_BR
dc.subject.otherlanguageCancer-associated fibroblastspt_BR
dc.description.abstractColorectal cancer (CRC) is in the top 10 cancers most prevalent worldwide, affecting equally men and women. Current research on tumor-derived extracellular vesicles (EVs) suggests that these small extracellular vesicles (sEVs) play an important role in mediating cell-to-cell communication and thus potentially affecting cancer progression via multiple pathways. In the present study, we hypothesized that sEVs derived from different CRC cell lines differ in their ability to reprogram normal human fibroblasts through a process called tumor education. The sEVs derived from CRC cell lines (HT29 and HCT116) were isolated by a combination of ultrafiltration and polymeric precipitation, followed by characterization based on morphology, size, and the presence or absence of EV and non-EV markers. It was observed that the HT29 cells displayed a higher concentration of sEVs compared with HCT116 cells. For the first time, we demonstrated that HT29-derived sEVs were positive for low-molecular-weight protein tyrosine phosphatase (Lmwptp). CRC cell-derived sEVs were uptake by human fibroblasts, stimulating migratory ability via Rho-Fak signaling in co-incubated human fibroblasts. Another important finding showed that HT29 cell-derived sEVs are much more efficient in activating human fibroblasts to cancer-associated fibroblasts (CAFs). Indeed, the sEVs produced by the HT29 cells that are less responsive to a cytotoxic agent display higher efficiency in educating normal human fibroblasts by providing them advantages such as activation and migratory ability. In other words, these sEVs have an influence on the CRC microenvironment, in part, due to fibroblasts reprogrammingpt_BR
dc.relation.ispartofFrontiers in cell and developmental biologypt_BR
dc.relation.ispartofabbreviationFront. cell dev. biol.pt_BR
dc.publisher.cityLausannept_BR
dc.publisher.countrySuiçapt_BR
dc.publisherFrontiers Research Foundationpt_BR
dc.date.issued2021-
dc.language.isoengpt_BR
dc.description.volume9pt_BR
dc.rightsAbertopt_BR
dc.identifier.eissn2296-634Xpt_BR
dc.identifier.doi10.3389/fcell.2021.696373pt_BR
dc.identifier.urlhttps://www.frontiersin.org/articles/10.3389/fcell.2021.696373/fullpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber2015/20412-7; 2018/03593-6pt_BR
dc.date.available2021-07-29T20:07:09Z-
dc.date.accessioned2021-07-29T20:07:09Z-
dc.description.provenanceSubmitted by Mariana Aparecida Azevedo (mary1@unicamp.br) on 2021-07-29T20:07:08Z No. of bitstreams: 1 DB27BEB7-CBAB.pdf: 3583847 bytes, checksum: e61705015aa1faa22325e40651e1e285 (MD5)en
dc.description.provenanceMade available in DSpace on 2021-07-29T20:07:09Z (GMT). No. of bitstreams: 1 DB27BEB7-CBAB.pdf: 3583847 bytes, checksum: e61705015aa1faa22325e40651e1e285 (MD5) Previous issue date: 2021en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/364055-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Bioquímica e Biologia Tecidualpt_BR
dc.contributor.departmentDepartamento de Bioquímica e Biologia Tecidualpt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordColorectal cancerpt_BR
dc.subject.keywordSmall extracellular vesiclespt_BR
dc.subject.keywordTumor educationpt_BR
dc.subject.keywordLmwptppt_BR
dc.subject.keywordTumor microenvironmentpt_BR
dc.identifier.sourceDB27BEB7-CBABpt_BR
dc.creator.orcid0000-0003-4231-1213pt_BR
dc.creator.orcid0000-0003-3149-021Xpt_BR
dc.creator.orcid0000-0002-7911-6334pt_BR
dc.type.formArtigo de pesquisa originalpt_BR
dc.identifier.articleid696373pt_BR
Appears in Collections:IB - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
DB27BEB7-CBAB.pdf3.5 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.