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Type: Artigo
Title: Early protein restriction increases intra-islet GLP-1 production and pancreatic β-cell proliferation mediated by the β-catenin pathway
Author: Rosa-Santos, Chaiane Aline da
Rodrigues, Priscila da Costa
Silva, Luana Resende
Arantes, Vanessa Cristina
Reis, Marise Auxiliadora de Barros
Colodel, Edson Moleta
Damazo, Amílcar Sabino
Moura, Egberto Gaspar de
Carneiro, Everardo Magalhães
Latorraca, Márcia Queiroz
Abstract: In the present study, we investigated whether intra-islet GLP-1 production and its modulation have a role in apoptosis, proliferation or neogenesis that is compromised by protein restriction during the foetal and suckling periods. Exendin-4, a GLP-1 receptor agonist (treated groups), or saline (non-treated groups) was intraperitoneally administered for 15 days from 75 to 90 days of age in female adult rats consisting of offspring born to and suckled by mothers fed a control diet (control groups) and who had the same diet until 90 days of age or offspring born to and suckled by mothers fed a low-protein diet and who were fed the control diet after weaning until 90 days of age (protein-restricted group). The β-cell mass was lower in the protein-restricted groups than in the control groups. Exendin-4 increased β-cell mass, regardless of the mother’s protein intake. The colocalization of GLP-1/glucagon was higher in the protein-restricted rats than in control rats in both the exendin-4-treated and non-treated groups. The frequency of cleaved caspase-3-labelled cells was higher in the non-treated protein-restricted group than in the non-treated control group and was similar in the treated protein-restricted and treated control groups. Regardless of treatment with exendin-4, Ki67-labelled cell frequency and β-catenin/DAPI colocalization were elevated in the protein-restricted groups. Exendin-4 increased the area of endocrine cell clusters and β-catenin/DAPI and FoxO1/DAPI colocalization regardless of the mother’s protein intake. Protein restriction in early life increased intra-islet GLP-1 production and β-cell proliferation, possibly mediated by the β-catenin pathway
Subject: Apoptose
Country: Alemanha
Editor: Springer
Rights: Fechado
Identifier DOI: 10.1007/s00394-020-02192-6
Date Issue: 2020
Appears in Collections:IB - Artigos e Outros Documentos

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