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Type: Artigo
Title: Tissue factor-dependent coagulation activation by heme: A thromboelastometry study
Author: de Souza, Gleice Regina
Hounkpe, Bidossessi Wilfried
Luz Fiusa, Maiara Marx
Colella, Marina Pereira
Annichino-Bizzacchi, Joyce M.
Traina, Fabiola
Costa, Fernando Ferreira
De Paula, Erich Vinicius
Abstract: Heme has been characterized as potent trigger of inflammation. In hemostasis, although heme has been shown to both induce and inhibit different compartments of hemostasis, its net effect on the hemostatic balance, and the biological relevance of these effects remain to be determined. Herein we evaluated the effect of heme on hemostasis using a global assay able to generate clinically relevant data in several other complex hemostatic diseases. Citrated whole blood samples from healthy participants were stimulated by heme or vehicle and incubated for 4h at 37 degrees C. Rotational thromboelastometry was immediately performed. The participation of tissue factor in coagulation activation was evaluated using inhibitory antibody. Heme was able of inducing ex vivo coagulation activation in whole blood, affecting predominantly parameters associated with the initial phases of clot formation. This activation effect was at least partially dependent on hematopoietic tissue factor, since the effects of heme were partially abrogated by the inhibition of human tissue factor. In conclusion, using a global hemostasis assay, our study confirmed that heme is able to activate coagulation in whole blood, in a tissue factor-dependent way. These findings could explain the disturbance in hemostatic balance observed in conditions associated with the release of heme such as sickle cell disease
Subject: Heme
Country: Estados Unidos
Editor: Public Library of Science
Rights: Aberto
Identifier DOI: 10.1371/journal.pone.0176505
Date Issue: 2017
Appears in Collections:FCM - Artigos e Outros Documentos
HEMO - Artigos e Outros Documentos

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