Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/36041
Type: Artigo de periódico
Title: Extraction of lipase from Burkholderia cepacia by PEG/Phosphate ATPS and its biochemical characterization
Author: Padilha, Giovana da Silva
Santana, José Carlos Curvelo
Alegre, Ranulfo Monte
Tambourgi, Elias Basile
Abstract: This work aimed to study the partitioning of a lipase produced by Burkholderia cepacia in PEG/Phosphate aqueous two phase system (ATPS) and its characterization. Lipase was produced by B. cepacia strains in a fermenter. Enzyme partitioning occurred at pH 6.0 and 8.0, using PEG 1500 and 6000 on two tie lines. Metal ions, pH and temperature effects on enzyme activity were evaluated. Five milliliter of 7.5% olive oil emulsion with 2.5% gumarabic in 0.1M sodium phosphate buffer at pH 8.0 and 37ºC were used for the activity determinations. Results showed that crude stratum from B. cepacia was partitioned by PEG1500/phosphate ATPS at pH 6.0 or 8.0 for, which the partitioning coefficients were 108-and 209-folds. Lipase presented optimal activity conditions at 37ºC and pH 8.0; it showed pH-stability for 4 h of incubation at different pH values at 37ºC. Metal ions such as Mn2+ , Co2+, I-and Ca2+ sustained enzymatic activities; however, it was inhibited by the presence of Fe2+, Hg2+ and Al3+ . Km and Vmax values were 0.258 U/mg and 43.90 g/L, respectively. A molecular weight of 33 kDa and an isoelectric point at pH 5.0 were determined by SDS-PAGE and IFS electrophoresis, respectively.
Subject: lipase
Burkholderia cepacia
aqueous two phase system
partitioning
characterization
Editor: Instituto de Tecnologia do Paraná - Tecpar
Rights: aberto
Identifier DOI: 10.1590/S1516-89132012000100002
Address: http://dx.doi.org/10.1590/S1516-89132012000100002
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100002
Date Issue: 1-Feb-2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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