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Type: Artigo
Title: Detrimental effect of fungal 60-kDa heat shock protein on experimental Paracoccidioides brasiliensis Infection
Author: Fernandes, Fabr-Cio Freitas
de Oliveira, Leandro Licursi
Landgraf, Leandro Licursi Taise Natali
Peron, Gabriela
Costa, Marcelo Vieira
Coelho-Castelo, Arlete A. M.
Bonato, Vania L. D.
Roque-Barreira, Maria-Cristina
Panunto-Castelo, Ademilson
Abstract: The genus Paracoccidioides comprises species of dimorphic fungi that cause paracocci-dioidomycosis (PCM), a systemic disease prevalent in Latin America. Here, we investigated whether administration of native 60-kDa heat shock protein of P. brasiliensis (nPbHsp60) or its recombinant counterpart (rPbHsp60) affected the course of experimental PCM. Mice were subcutaneously injected with nPbHsp60 or rPbHsp60 emulsified in complete's Freund Adjuvant (CFA) at three weeks after intravenous injection of P. brasiliensis yeasts. Infected control mice were injected with CFA or isotonic saline solution alone. Thirty days after the nPbHsp60 or rPbHsp60 administration, mice showed remarkably increased fungal load, tissue inflammation, and granulomas in the lungs, liver, and spleen compared with control mice. Further, rPbHsp60 treatment (i) decreased the known protective effect of CFA against PCM and (ii) increased the concentrations of IL-17, TNF-alpha, IL-12, IFN-gamma, IL-4, IL-10, and TGF-beta in the lungs. Together, our results indicated that PbHsp60 induced a harmful immune response, exacerbated inflammation, and promoted fungal dissemination. Therefore, we propose that PbHsp60 contributes to the fungal pathogenesis
Subject: Paracoccidioides brasiliensis
Country: Estados Unidos
Editor: Public Library of Science
Rights: Aberto
Identifier DOI: 10.1371/journal.pone.0162486
Date Issue: 2016
Appears in Collections:IB - Artigos e Outros Documentos

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