Please use this identifier to cite or link to this item:
Type: Artigo
Title: Effects of microcurrent therapy on excisional elastic cartilage defects in young rats
Author: Tangerino Filho, Edson Pereira
Fachi, Jose Luis
Vasconcelos, Israel Costa
Tech dos Santos, Glaucia Maria
Sampaio Mendonca, Fernanda Aparecida
de Aro, Andrea Aparecida
Pimentel, Edson Rosa
Marretto Esquisatto, Marcelo Augusto
Abstract: The effects of microcurrent application on the elastic cartilage defects in the outer ear of young animals were analyzed. Sixty male Wistar rats were divided into a control (CG) and a treated group (TG). An excisional lesion was created in the right outer ear of each animal. Daily treatment was started after 24 hand consisted of the application of a low-intensity (20 mu A) continuous electrical current to the site of injury for 5 min. The animals were euthanized after 7, 14 and 28 days of injury and the samples were submitted to analyses. In CG, areas of newly formed cartilage and intense basophilia were seen at 28 days, while in TG the same observations were made already at 14 days. The percentage of birefringent collagen fibers was higher in CG at 28 days. The number of connective tissue cells and granulocytes was significantly higher in TG. Ultrastructural analysis revealed the presence of chondrocytes in TG at 14 days, while these cells were observed in CG only at 28 days. Cuprolinic blue staining and the amount of glycosaminoglycans were significantly higher in TG at 14 days and 28 days. The amount of hydroxyproline was significantly higher in TG at all time points studied. The active isoform of MMP-2 was higher activity in TG at 14 days. Immunoblotting for type II collagen and decorin was positive in both groups and at all time points. The treatment stimulated the proliferation and differentiation of connective tissue cells, the deposition of glycosaminoglycans and collagen, and the structural reorganization of these elements during elastic cartilage repair
Subject: Colágeno
Country: Reino Unido
Editor: Elsevier
Rights: Fechado
Identifier DOI: 10.1016/j.tice.2016.03.004
Date Issue: 2016
Appears in Collections:IB - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
000376396200010.pdf5.17 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.