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|Title:||In vitro incubation of human spermatozoa promotes reactive oxygen species generation and DNA fragmentation|
|Abstract:||The aim of this study was to investigate the oxidative process associated with sperm capacitation and its impact on DNA fragmentation and sperm function. Redox activity and lipid peroxidation were analysed in human spermatozoa after 3, 6 and 22h of incubation in Hams F10 medium plus bovine albumin at 37 degrees and 5% CO2 for capacitation. DNA status, tyrosine phosphorylation pattern and induced acrosome reaction were evaluated after capacitating conditions. At 22h of incubation, there was a significant (P<0.05) increase in oxygen-free radicals and lipid peroxidation, with no effect on sperm viability. There also was a significant (P<0.001) increase in fragmented DNA in capacitated spermatozoa compared to semen values with higher rates being found after the occurrence of the induced acrosome reaction. Protein tyrosine phosphorylation pattern confirms that capacitation took place in parallel with the occurrence of DNA fragmentation. These results indicate that when spermatozoa are incubated for several hours (22h), a common practice in assisted reproductive techniques, an increase in oxidative sperm metabolism and in the proportion of fragmentedDNA should be expected. However, there was no effect on any of the other functional parameters associated with sperm fertilising capacity|
|Subject:||Fragmentação do DNA|
|Editor:||Wiley-Blackwell Verlag GmbH|
|Appears in Collections:||FCM - Artigos e Outros Documentos|
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