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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampStipp, Rafael Nobrega-
dc.typeArtigopt_BR
dc.titleInsights into the virulence traits of streptococcus mutans in dentine carious lesions of children with early childhood cariespt_BR
dc.contributor.authorBezerra, Daniela S.-
dc.contributor.authorStipp, Rafael N.-
dc.contributor.authorNeves, Beatriz G.-
dc.contributor.authorGuedes, Sarah F. F.-
dc.contributor.authorNascimento, Marcelle M.-
dc.contributor.authorRodrigues, Lidiany K. A.-
dc.subjectExpressão gênicapt_BR
dc.subject.otherlanguageGene expressionpt_BR
dc.description.abstractStreptococcus mutans is an oral bacterium considered to play a major role in the development of dental caries. This study aimed to investigate the prevalence of S. mutans in active and arrested dentine carious lesions of children with early childhood caries and to examine the expression profile of selected S. mutans genes associated with survival and virulence, within the same carious lesions. Dentine samples were collected from 29 active and 16 arrested carious lesions that were diagnosed in preschool children aged 2-5 years. Total RNA was extracted from the dentine samples, and reverse transcription quantitative real-time PCR analyses were performed for the quantification of S. mutans and for analyses of the expression of S. mutans genes associated with bacterial survival (atpD, nox, pdhA) and virulence (fabM and aguD). There was no statistically significant difference in the prevalence of S. mutans between active and arrested carious lesions. Expression of the tested genes was detected in both types of carious dentine. The pdhA (p = 0.04) and aguD (p = 0.05) genes were expressed at higher levels in arrested as compared to active lesions. Our findings revealed that S. mutans is part of the viable microbial community in active and arrested dentine carious lesions. The increase in expression of the pdhA and aguD genes in arrested lesions is likely due to the unfavourable environmental conditions for microbial growth, inherent to this type of lesionspt_BR
dc.relation.ispartofCaries researchpt_BR
dc.relation.ispartofabbreviationCaries res.pt_BR
dc.publisher.cityBaselpt_BR
dc.publisher.countrySuiçapt_BR
dc.publisherKargerpt_BR
dc.date.issued2016-
dc.language.isoengpt_BR
dc.description.volume50pt_BR
dc.description.issuenumber3pt_BR
dc.description.firstpage279pt_BR
dc.description.lastpage287pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0008-6568pt_BR
dc.identifier.eissn1421-976Xpt_BR
dc.identifier.doi10.1159/000445256pt_BR
dc.identifier.urlhttps://www.karger.com/Article/FullText/445256pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsordocumentnumber475346/2011-4-MCT/CNPq14/2011-Universalpt_BR
dc.date.available2021-02-18T12:16:18Z-
dc.date.accessioned2021-02-18T12:16:18Z-
dc.description.provenanceSubmitted by Sanches Olivia (olivias@unicamp.br) on 2021-02-18T12:16:18Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-05-25T12:08:38Z : No. of bitstreams: 1 000378789700002.pdf: 297487 bytes, checksum: c8edc2526f6aa99a9ba16e05b8912b43 (MD5)en
dc.description.provenanceMade available in DSpace on 2021-02-18T12:16:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2016en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/356047-
dc.contributor.departmentDepartamento de Diagnóstico Oralpt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.subject.keywordDentine cariespt_BR
dc.subject.keywordEarly childhood cariespt_BR
dc.subject.keywordStreptococcus mutanspt_BR
dc.identifier.source000378789700002pt_BR
dc.creator.orcid0000-0001-5305-1299pt_BR
dc.type.formArtigo originalpt_BR
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