Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/355982
Type: Artigo
Title: A linker of the proline-threonine repeating motif sequence is bimodal
Author: Skaf, Munir Salomão
Polikarpov, Igor
Stanković, Ivana M.
Abstract: The linker of the endoglucanase from Xanthomonas campestris pv. campestris ((PT)12) has a specific sequence, a repeating proline-threonine motif. In order to understand its role, it has been compared to a regular sequence linker, in this work—the cellobiohydrolase 2 from Trichoderma reesei (CBH2). Elastic properties of the two linkers have been estimated by calculating free energy profile along the linker length from an enhanced sampling molecular dynamics simulation. The (PT)12 exhibits more pronounced elastic behaviour than CBH2. The PT repeating motif results in a two-mode energy profile which could be very useful in the enzyme motions along the substrate during hydrolytic catalysis
Subject: Dinâmica molecular
Endoglucanases
Country: Alemanha
Editor: Springer
Rights: Fechado
Identifier DOI: 10.1007/s00894-020-04434-0
Address: https://link.springer.com/article/10.1007%2Fs00894-020-04434-0
Date Issue: 2020
Appears in Collections:IQ - Artigos e Outros Documentos

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