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Type: Artigo
Title: A historical review of the techniques of recovery of parasites for their detection in human stools
Author: Soares, Felipe Augusto
Benitez, Aline do Nascimento
Santos, Bianca Martins dos
Loiola, Saulo Hudson Nery
Rosa, Stefany Laryssa
Nagata, Walter Bertequini
Inácio, Sandra Valéria
Suzuki, Celso Tetsuo Nagase
Bresciani, Katia Denise Saraiva
Falcão, Alexandre Xavier
Gomes, Jancarlo Ferreira
Abstract: Since the early 20th century, the detection of intestinal parasites has improved with the development of several techniques for parasitic structures recovery and identification, which differ in sensitivity, specificity, practicality, cost, and infrastructure demand. This study aims to review, in chronological order, the stool examination techniques and discuss their advantages, limitations, and perspectives, and to provide professionals and specialists in this field with data that lays a foundation for critical analysis on the use of such procedures. The concentration procedures that constitute the main techniques applied in routine research and in parasitological kits are a) spontaneous sedimentation; b) centrifugation-sedimentation with formalin-ethyl acetate; and c) flotation with zinc sulfate solution. While selecting a technique, one should consider the purpose of its application and the technical-operational, biological, and physicochemical factors inherent in the procedures used in stool processing, which may restrict its use. These intrinsic limitations may have undergone procedural changes driven by scientific and technological development and by development of alternative methods, which now contribute to the improvement of diagnostic accuracy
Subject: Parasitologia
Country: Brasil
Editor: Sociedade Brasileira de Medicina Tropical
Rights: Aberto
Identifier DOI: 10.1590/0037-8682-0535-2019
Date Issue: 2020
Appears in Collections:IC - Artigos e Outros Documentos

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