Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/355871
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampBertól, Jéssica Wildgrube-
dc.contributor.authorunicampAlves, Maria Clara Duarte Fregolente-
dc.contributor.authorunicampCaruzo, Thabata Alessandra Ramos-
dc.contributor.authorunicampSilva, Márcio José da-
dc.contributor.authorunicampGatti, Maria Silvia Viccari-
dc.typeArtigopt_BR
dc.titleMolecular characterisation of the NSP4 gene of group A human rotavirus G2P[4] strains circulating in São Paulo, Brazil, from 1994 and 2006 to 2010pt_BR
dc.contributor.authorBertol, Jessica Wildgrube-
dc.contributor.authorDuarte Fregolente, Maria Clara-
dc.contributor.authorRamos Caruzo, Thabata Alessandra-
dc.contributor.authorda Silva, Marcio Jose-
dc.contributor.authorMunford, Veridiana-
dc.contributor.authorPalazzi Safadi, Marco Aurelio-
dc.contributor.authorRacz, Maria Lucia-
dc.contributor.authorViccari Gatti, Maria Silvia-
dc.subjectRotavíruspt_BR
dc.subject.otherlanguageRotaviruspt_BR
dc.description.abstractGroup A human rotaviruses (HuRVA) are causative agents of acute gastroenteritis. Six viral structural proteins (VPs) and six nonstructural proteins (NSPs) are produced in RV-infected cells. NSP4 is a diarrhoea-inducing viral enterotoxin and NSP4 gene analysis revealed at least 15 (E1-E15) genotypes. This study analysed the NSP4 genetic diversity of HuRVA G2P[4] strains collected in the state of Sao Paulo (SP) from 1994 and 2006-2010 using reverse transcription-polymerase chain reaction, sequencing and phylogenetic analysis. Forty (97.6%) G2P[4] strains displayed genotype E2; one strain (2.4%) displayed genotype E1. These results are consistent with the proposed linkage between VP4/VP7 (G2P[4]) and the NSP4 (E2) genotype of HuRVA. NSP4 phylogenetic analysis showed distinct clusters, with grouping of most strains by their genotype and collection year, and most strains from SP were clustered together with strains from other Brazilian states. A deduced amino acid sequence alignment for E2 showed many variations in the C-terminal region, including the VP4-binding domain. Considering the ability of NSP4 to generate host immunity, monitoring NSP4 variations, along with those in the VP4 or VP7 protein, is important for evaluating the circulation and pathogenesis of RV. Finally, the presence of one G2P[4] E1 strain reinforces the idea that new genotype combinations emerge through reassortment and independent segregationpt_BR
dc.relation.ispartofMemórias do Instituto Oswaldo Cruzpt_BR
dc.relation.ispartofabbreviationMem. Inst. Oswaldo Cruzpt_BR
dc.publisher.cityRio de Janeiro, RJpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisherFIOCRUZpt_BR
dc.date.issued2015-
dc.date.monthofcirculationSep.pt_BR
dc.language.isoengpt_BR
dc.description.volume110pt_BR
dc.description.issuenumber6pt_BR
dc.description.firstpage786pt_BR
dc.description.lastpage792pt_BR
dc.rightsAbertopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0074-0276pt_BR
dc.identifier.eissn0074-0276pt_BR
dc.identifier.doi10.1590/0074-02760150199pt_BR
dc.identifier.urlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000600786pt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber2009/08685-7; 2011/13725-8pt_BR
dc.date.available2021-02-16T10:13:28Z-
dc.date.accessioned2021-02-16T10:13:28Z-
dc.description.provenanceSubmitted by Sanches Olivia (olivias@unicamp.br) on 2021-02-16T10:13:28Z No. of bitstreams: 0en
dc.description.provenanceMade available in DSpace on 2021-02-16T10:13:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2015en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/355871-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Genética, Evolução e Bioagentespt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeCentro de Biologia Molecular e Engenharia Genéticapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordNSP4 genotypespt_BR
dc.subject.keywordPhylogenetic analysispt_BR
dc.subject.keywordVP4/VP7/NSP4 linkagept_BR
dc.subject.keywordBrazilpt_BR
dc.identifier.source000364428100011pt_BR
dc.creator.orcid0000-0002-0898-1822pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0001-9573-1655pt_BR
dc.creator.orcid0000-0003-3820-9726pt_BR
dc.creator.orcid0000-0003-3614-5503pt_BR
dc.type.formArtigopt_BR
Appears in Collections:IB - Artigos e Outros Documentos
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