Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/355775
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampAngelis, Célio Damacena de-
dc.typeArtigopt_BR
dc.titleA comprehensive time course study of tissue nitric oxide metabolites concentrations after oral nitrite administrationpt_BR
dc.contributor.authorPinheiro, Lucas C.-
dc.contributor.authorFerreira, Graziele C.-
dc.contributor.authorAngelis, Célio Damacena de-
dc.contributor.authorToledo Jr., Jose Carlos-
dc.contributor.authorTanus-Santos, Jose E.-
dc.subjectNitritospt_BR
dc.subject.otherlanguageNitritespt_BR
dc.description.abstractNitrite and nitrate are considered nitric oxide (NO) storage pools. The assessment of their tissue concentrations may improve our understanding of how they attenuate pathophysiological mechanisms promoting disease. We hypothesized that significant differences exist when the tissue concentrations of nitrite, nitrate, and nitrosylated species (RXNO) are compared among different tissues, particularly when nitrite is administered orally because nitrite generates various NO-related species in the stomach. We studied the different time-dependent changes in plasma and tissue concentrations of nitrite, nitrate, and RXNO after oral nitrite 15 mg/kg was administered rats, which were euthanized 15, 30, 60, 120, 240, 480 or 1440 min after nitrite administration. A control group received water. Arterial blood samples were collected and the rats were perfused with a PBS solution containing NEM/DTPA to prevent the destruction of RXNO. After perfusion, heart, aorta, mesenteric artery, brain, stomach, liver and femoral muscle were harvested and immediately stored at −70°C until analyzed for their nitrite, nitrate and RXNO contents using an ozone-based reductive chemiluminescence assay. While nitrite administration did not increase aortic nitrite or nitrate concentrations for at least 60 min, both aorta and mesenteric vessels stored nitrite from 8 to 24 h after its administration and their tissue concentrations increased from 10 to 40-fold those found in plasma. In contrast, the other studied tissues showed only transient increases in the concentrations of these NO metabolites, including RXNO. The differences among tissues may reflect differences in mechanisms regulating cellular influx of nitrite. These findings have important pharmacological and clinical implicationspt_BR
dc.relation.ispartofFree radical biology and medicinept_BR
dc.relation.ispartofabbreviationFree radic. biol. med.pt_BR
dc.publisher.cityPhiladelphia, PApt_BR
dc.publisher.countryEstados Unidospt_BR
dc.publisherElsevierpt_BR
dc.date.issued2020-
dc.date.monthofcirculationMay.pt_BR
dc.language.isoengpt_BR
dc.description.volume152pt_BR
dc.description.firstpage43pt_BR
dc.description.lastpage51pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0891-5849pt_BR
dc.identifier.eissn1873-4596pt_BR
dc.identifier.doi10.1016/j.freeradbiomed.2020.03.006pt_BR
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0891584919323949pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsordocumentnumberNão tempt_BR
dc.description.sponsordocumentnumber001pt_BR
dc.description.sponsordocumentnumber2014-23946-0pt_BR
dc.date.available2021-02-15T13:00:41Z-
dc.date.accessioned2021-02-15T13:00:41Z-
dc.description.provenanceSubmitted by Susilene Barbosa da Silva (susilene@unicamp.br) on 2021-02-15T13:00:41Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-05-24T19:06:54Z : No. of bitstreams: 1 000542948900005.pdf: 2018800 bytes, checksum: 68b01a9c3d0cec07342a9826b9e98b67 (MD5)en
dc.description.provenanceMade available in DSpace on 2021-02-15T13:00:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2020en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/355775-
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.subject.keywordNitrosylated speciespt_BR
dc.identifier.source000542948900005pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.type.formArtigopt_BR
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