Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/355619
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampAro, Andrea Aparecida de-
dc.contributor.authorunicampGomes, Laurecir-
dc.contributor.authorunicampPimentel, Edson Rosa-
dc.typeArtigopt_BR
dc.titleStructure and composition of arytenoid cartilage of the bullfrog (Lithobates catesbeianus) during maturation and agingpt_BR
dc.contributor.authordos Santos Laureano, Priscila Eliane-
dc.contributor.authorSilva Oliveira, Kris Daiana-
dc.contributor.authorde Aro, Andrea Aparecida-
dc.contributor.authorGomes, Laurecir-
dc.contributor.authorPimentel, Edson Rosa-
dc.contributor.authorMarretto Esquisatto, Marcelo Augusto-
dc.subjectRã touropt_BR
dc.subject.otherlanguageBullfrogpt_BR
dc.description.abstractThe aging process induces progressive and irreversible changes in the structural and functional organization of animals. The objective of this study was to evaluate the effects of aging on the structure and composition of the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes. Animals at 7, 180 and 1080 days post-metamorphosis (n = 10/age) were euthanized and the cartilage was removed and processed for structural and biochemical analysis. For the structural analyses, cartilage sections were stained with picrosirius, toluidine blue, Weigert's resorcin-fuchsin and Von Kossa stain. The sections were also submitted to immunohistochemistry for detection of collagen types I and II. Other samples were processed for the ultrastructural and cytochemical analysis of proteoglycans. Histological sections were used to chondrocyte count. The number of positive stainings for proteoglycans was quantified by ultrastructural analysis. For quantification and analysis of glycosaminoglycans were used the dimethyl methylene blue and agarose gel electrophoresis methods. The chloramine T method was used for hydroxyproline quantification. At 7 days, basophilia was observed in the pericellular and territorial matrix, which decreased in the latter over the period studied. Collagen fibers were arranged perpendicular to the major axis of the cartilaginous plate and were thicker in older animals. Few calcification areas were observed at the periphery of the cartilage specimens in 1080-day-old animals. Type II collagen was present throughout the stroma at the different ages. Elastic fibers were found in the stroma and perichondrium and increased with age in the two regions. Proteoglycan staining significantly increased from 7 to 180 days and reduced at 1080 days. The amount of total glycosaminoglycans was higher in 180-day-old animals compared to the other ages, with marked presence of chondroitin- and dermatan-sulfate especially in this age. The content of hydroxyproline, which infers the total collagen concentration, was higher in 1080-day-old animals compared to the other ages. The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix. These changes may contribute to alter the function of the larynx in the animal during agingpt_BR
dc.relation.ispartofMicron: the international research and review journal for microscopypt_BR
dc.relation.ispartofabbreviationMicronpt_BR
dc.publisher.cityNew York, NYpt_BR
dc.publisher.countryEstados Unidospt_BR
dc.publisherElsevierpt_BR
dc.date.issued2015-
dc.date.monthofcirculationOct.pt_BR
dc.language.isoengpt_BR
dc.description.volume77pt_BR
dc.description.firstpage16pt_BR
dc.description.lastpage24pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0968-4328pt_BR
dc.identifier.eissn1878-4291pt_BR
dc.identifier.doi10.1016/j.micron.2015.05.018pt_BR
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0968432815300135pt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumbersem informaçãopt_BR
dc.date.available2021-02-11T16:25:34Z-
dc.date.accessioned2021-02-11T16:25:34Z-
dc.description.provenanceSubmitted by Sanches Olivia (olivias@unicamp.br) on 2021-02-11T16:25:34Z No. of bitstreams: 0en
dc.description.provenanceMade available in DSpace on 2021-02-11T16:25:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2015en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/355619-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Biologia Estrutural e Funcionalpt_BR
dc.contributor.departmentDepartamento de Biologia Estrutural e Funcionalpt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordCartilagept_BR
dc.subject.keywordStructurept_BR
dc.subject.keywordLarynxpt_BR
dc.subject.keywordAgingpt_BR
dc.identifier.source000360420700003pt_BR
dc.creator.orcid0000-0003-2895-0000pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0002-1049-8880pt_BR
dc.type.formArtigopt_BR
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