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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampMartins-de-Souza, Daniel-
dc.typeArtigopt_BR
dc.titleMK-801 treatment affects glycolysis in oligodendrocytes more than in astrocytes and neuronal cells : insights for schizophreniapt_BR
dc.contributor.authorGuest, Paul C.-
dc.contributor.authorIwata, Keiko-
dc.contributor.authorKato, Takahiro A.-
dc.contributor.authorSteiner, Johann-
dc.contributor.authorSchmitt, Andrea-
dc.contributor.authorW.Turck, Christoph-
dc.contributor.authorMartins-de-Souza, Daniel-
dc.subjectGlicólisept_BR
dc.subject.otherlanguageGlycolysispt_BR
dc.description.abstractSchizophrenia is a debilitating mental disorder, affecting more than 30 million people worldwide. As a multifactorial disease, the underlying causes of schizophrenia require analysis by multiplex methods such as proteomics to allow identification of whole protein networks. Previous post-mortem proteomic studies on brain tissues from schizophrenia patients have demonstrated changes in activation of glycolytic and energy metabolism pathways. However, it is not known whether these changes occur in neurons or in glial cells. To address this question, we treated neuronal, astrocyte, and oligodendrocyte cell lines with the NMDA receptor antagonist MK-801 and measured the levels of six glycolytic enzymes by Western blot analysis. MK-801 acts on the glutamatergic system and has been proposed as a pharmacological means of modeling schizophrenia. Treatment with MK-801 resulted in significant changes in the levels of glycolytic enzymes in all cell types. Most of the differences were found in oligodendrocytes, which had altered levels of hexokinase 1 (HK1), enolase 2 (ENO2), phosphoglycerate kinase (PGK), and phosphoglycerate mutase 1 after acute MK-801 treatment (8 h), and HK1, ENO2, PGK, and triosephosphate isomerase (TPI) following long term treatment (72 h). Addition of the antipsychotic clozapine to the cultures resulted in counter-regulatory effects to the MK-801 treatment by normalizing the levels of ENO2 and PGK in both the acute and long term cultures. In astrocytes, MK-801 affected only aldolase C (ALDOC) under both acute conditions and HK1 and ALDOC following long term treatment, and TPI was the only enzyme affected under long term conditions in the neuronal cells. In conclusion, MK-801 affects glycolysis in oligodendrocytes to a larger extent than neuronal cells and this may be modulated by antipsychotic treatment. Although cell culture studies do not necessarily reflect the in vivo pathophysiology and drug effects within the brain, these results suggest that neurons, astrocytes, and oligodendrocytes are affected differently in schizophrenia. Employing in vitro models using neurotransmitter agonists and antagonists may provide new insights about the pathophysiology of schizophrenia which could lead to a novel system for drug discoverypt_BR
dc.relation.ispartofFrontiers in cellular neurosciencept_BR
dc.relation.ispartofabbreviationFront. cell. neurosci.pt_BR
dc.publisher.cityLausannept_BR
dc.publisher.countrySuiçapt_BR
dc.publisherFrontiers Research Foundationpt_BR
dc.date.issued2015-
dc.date.monthofcirculationMay.pt_BR
dc.language.isoengpt_BR
dc.rightsAbertopt_BR
dc.sourceWOSpt_BR
dc.identifier.eissn1662-5102pt_BR
dc.identifier.doi10.3389/fncel.2015.00180pt_BR
dc.identifier.urlhttps://www.frontiersin.org/articles/10.3389/fncel.2015.00180/pt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber13/08711-3pt_BR
dc.date.available2021-02-08T19:31:29Z-
dc.date.accessioned2021-02-08T19:31:29Z-
dc.description.provenanceSubmitted by Susilene Barbosa da Silva (susilene@unicamp.br) on 2021-02-08T19:31:29Z No. of bitstreams: 0en
dc.description.provenanceMade available in DSpace on 2021-02-08T19:31:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2015en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/355372-
dc.contributor.departmentDepartamento de Bioquímica e Biologia Tecidualpt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.identifier.source000354803600002pt_BR
dc.creator.orcid0000-0003-3595-5846pt_BR
dc.type.formArtigo de pesquisapt_BR
dc.description.sponsorNoteFAEPEX UNICAMP grant number 519.292pt_BR
Appears in Collections:IB - Artigos e Outros Documentos

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