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Type: Artigo
Title: Compositional profile of α / β‐hydrolase fold proteins in mangrove soil metagenomes: prevalence of epoxide hydrolases and haloalkane dehalogenases in oil‐contaminated sites
Author: Jiménez, Diego Javier
Dini‐Andreote, Francisco
Ottoni, Júlia Ronzella
Oliveira, Valéria Maia de
Elsas, Jan Dirk van
Andreote, Fernando Dini
Abstract: The occurrence of genes encoding biotechnologically relevant α/β‐hydrolases in mangrove soil microbial communities was assessed using data obtained by whole‐metagenome sequencing of four mangroves areas, denoted BrMgv01 to BrMgv04, in São Paulo, Brazil. The sequences (215 Mb in total) were filtered based on local amino acid alignments against the Lipase Engineering Database. In total, 5923 unassembled sequences were affiliated with 30 different α/β‐hydrolase fold superfamilies. The most abundant predicted proteins encompassed cytosolic hydrolases (abH08; ∼ 23%), microsomal hydrolases (abH09; ∼ 12%) and Moraxella lipase‐like proteins (abH04 and abH01; < 5%). Detailed analysis of the genes predicted to encode proteins of the abH08 superfamily revealed a high proportion related to epoxide hydrolases and haloalkane dehalogenases in polluted mangroves BrMgv01‐02‐03. This suggested selection and putative involvement in local degradation/detoxification of the pollutants. Seven sequences that were annotated as genes for putative epoxide hydrolases and five for putative haloalkane dehalogenases were found in a fosmid library generated from BrMgv02 DNA. The latter enzymes were predicted to belong to Actinobacteria, Deinococcus‐Thermus, Planctomycetes and Proteobacteria. Our integrated approach thus identified 12 genes (complete and/or partial) that may encode hitherto undescribed enzymes. The low amino acid identity (< 60%) with already‐described genes opens perspectives for both production in an expression host and genetic screening of metagenomes
Subject: Proteínas
Country: Inglaterra
Editor: Wiley
Rights: Fechado
Identifier DOI: 10.1111/1751-7915.12157
Date Issue: 2015
Appears in Collections:IB - Artigos e Outros Documentos
CPQBA - Artigos e Outros Documentos

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