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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampPereira, Luis Antonio Violin Dias-
dc.typeArtigopt_BR
dc.titleVentilation causes pulmonary vascular dilation and modulates the NOS and VEGF pathway on newborn rats with CDHpt_BR
dc.contributor.authorGallindo, Rodrigo Melo-
dc.contributor.authorLanhellas, Frances Lilian-
dc.contributor.authorFigueira, Rebeca Lopes-
dc.contributor.authorPereira, Luís Antônio Violin Dias-
dc.contributor.authorSimões, Ana Leda Bertoncini-
dc.contributor.authorSchmidt, Augusto Frederico-
dc.contributor.authorSbragia, Lourenço-
dc.subjectVentilaçãopt_BR
dc.subject.otherlanguageVentilationpt_BR
dc.description.abstractCongenital diaphragmatic hernia (CDH) is a defect that presents high mortality because of pulmonary hypoplasia and hypertension. Mechanical ventilation changes signaling pathways, such as nitric oxide and VEGF in the pulmonary arterioles. We investigated the production of NOS2 and NOS3 and expression of VEGF and its receptors after ventilation in rat fetuses with CDH.CDH was induced by Nitrofen. The fetuses were divided into 6 groups: 1) control (C); 2) control ventilated (CV); 3) exposed to nitrofen (N −); 4) exposed to nitrofen ventilated (N-V), 5) CDH and 6) CDH ventilated (CDHV). Fetuses were harvested and ventilated. We assessed body weight (BW), total lung weight (TLW), TLW/BW ratio, the median pulmonary arteriolar wall thickness (MWT). We analyzed the expression of NOS2, NOS3, VEGF and its receptors by immunohistochemistry and Western blotting. BW, TLW, and TLW/BW ratio were greater on C than on N − and CDH (p < 0.05). The MWT was higher in CDH than in CDHV (p < 0.001). CDHV showed increased expression of NOS3 (p < 0.05) and VEGFR1 (p < 0.05), but decreased expression of NOS2 (p < 0.05) and VEGFR2 (p < 0.001) compared to CDH.Ventilation caused pulmonary vasodilation and changed the expression of NOS and VEGF receptorspt_BR
dc.relation.ispartofJournal of pediatric surgerypt_BR
dc.relation.ispartofabbreviationJ Pediatr Surgpt_BR
dc.publisher.cityPhiladelphia, PApt_BR
dc.publisher.countryEstados Unidospt_BR
dc.publisherElsevierpt_BR
dc.date.issued2015-
dc.date.monthofcirculationMay.pt_BR
dc.language.isoengpt_BR
dc.description.volume50pt_BR
dc.description.issuenumber5pt_BR
dc.description.firstpage842pt_BR
dc.description.lastpage848pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0022-3468pt_BR
dc.identifier.eissn1531-5037pt_BR
dc.identifier.doi10.1016/j.jpedsurg.2014.12.005pt_BR
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0022346814008021pt_BR
dc.date.available2021-02-08T14:33:51Z-
dc.date.accessioned2021-02-08T14:33:51Z-
dc.description.provenanceSubmitted by Susilene Barbosa da Silva (susilene@unicamp.br) on 2021-02-08T14:33:51Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-05-24T19:06:28Z : No. of bitstreams: 1 000353813300032.pdf: 1702434 bytes, checksum: 8ab7cfb9f9f778b51d9df2c38a108e3b (MD5)en
dc.description.provenanceMade available in DSpace on 2021-02-08T14:33:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2015en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/355340-
dc.contributor.departmentDepartamento de Bioquímica e Biologia Tecidualpt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.identifier.source000353813300032pt_BR
dc.creator.orcid0000-0002-9332-7285pt_BR
dc.type.formArtigopt_BR
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