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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampVerma, Renu-
dc.contributor.authorunicampMaluta, Renato Pariz-
dc.contributor.authorunicampDa Silva, Livia Pilatti Mendes-
dc.contributor.authorunicampNakazato, Gerson-
dc.typeArtigopt_BR
dc.titleFimbria-Encoding Gene yadC Has a Pleiotropic Effect on Several Biological Characteristics and Plays a Role in Avian Pathogenic Escherichia coli Pathogenicitypt_BR
dc.contributor.authorVerma, Renu-
dc.contributor.authorGalvao Rojas, Thas Cabrera-
dc.contributor.authorMaluta, Renato Pariz-
dc.contributor.authorLeite, Janana Luisa-
dc.contributor.authorMendes da Silva, Livia Pilatti-
dc.contributor.authorNakazato, Gerson-
dc.contributor.authorda Silveira, Wanderley Dias-
dc.subjectCélulas HeLapt_BR
dc.subjectFatores de virulênciapt_BR
dc.subject.otherlanguageVirulence factorspt_BR
dc.subject.otherlanguageHeLa cellspt_BR
dc.description.abstractThe extraintestinal pathogen termed avian pathogenic Escherichia coli (APEC) is known to cause colibacillosis in chickens. The molecular basis of APEC pathogenesis is not fully elucidated yet. In this work, we deleted a component of the Yad gene cluster (yadC) in order to understand the role of Yad in the pathogenicity of the APEC strain SCI-07. In vitro, the transcription level of yadC was upregulated at 41 degrees C and downregulated at 22 degrees C. The yadC expression in vivo was more pronounced in lungs than in spleen, suggesting a role in the early steps of the infection. Chicks infected with the wild-type and mutant strains presented, respectively, 80% and 50% mortality rates. The Delta yadC strain presented a slightly decreased ability to adhere to HeLa cells with or without the D-mannose analog compared with the wild type. Real-time PCR (RT-PCR) assays showed that fimH was downregulated (P< 0.05) and csgA and ecpA were slightly upregulated in the mutant strain, showing that yadC modulates expression of other fimbriae. Bacterial internalization studies showed that the Delta yadC strain had a lower number of intracellular bacteria recovered from Hep-2 cells and HD11 cells than the wild-type strain (P< 0.05). Motility assays in soft agar demonstrated that the Delta yadC strain was less motile than the wild type (P< 0.01). Curiously, flagellum-associated genes were not dramatically downregulated in the Delta yadC strain. Taken together, the results show that the fimbrial adhesin Yad contributes to the pathogenicity and modulates different biological characteristics of the APEC strain SCI-07pt_BR
dc.relation.ispartofInfection and Immunitypt_BR
dc.relation.ispartofabbreviationIAIpt_BR
dc.publisher.cityWashington, DCpt_BR
dc.publisher.countryEstados Unidospt_BR
dc.publisherAmerican Society for Microbiologypt_BR
dc.date.issued2015-
dc.date.monthofcirculationJan.pt_BR
dc.language.isoengpt_BR
dc.description.volume84pt_BR
dc.description.issuenumber1pt_BR
dc.description.firstpage187pt_BR
dc.description.lastpage93pt_BR
dc.rightsAbertopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn1098-5522pt_BR
dc.identifier.eissn0019-9567pt_BR
dc.identifier.doi10.1128/IAI.01138-15pt_BR
dc.identifier.urlhttps://iai.asm.org/content/84/1/187.shortpt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumbersem informaçãopt_BR
dc.description.sponsordocumentnumber2012/23289-3; 2013/09167-5; 2012/05073-3; 2012/04391-1; 2014/04860-7pt_BR
dc.date.available2021-02-05T19:39:20Z-
dc.date.accessioned2021-02-05T19:39:20Z-
dc.description.provenanceSubmitted by Cintia Oliveira de Moura (cintiaom@unicamp.br) on 2021-02-05T19:39:20Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-05-25T14:38:14Z : No. of bitstreams: 1 000367187200009.pdf: 1012428 bytes, checksum: bf6ea751325ebfa362ac777526bf7a92 (MD5)en
dc.description.provenanceMade available in DSpace on 2021-02-05T19:39:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2015en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/355312-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.identifier.source000367187200009pt_BR
dc.creator.orcid0000-0003-1277-8824pt_BR
dc.creator.orcid0000-0003-3249-5127pt_BR
dc.creator.orcid0000-0003-4306-5413pt_BR
dc.creator.orcid0000-0001-8624-795Xpt_BR
dc.type.formArtigopt_BR
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