Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/355295
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampLander, Noelia Marina-
dc.contributor.authorunicampSiervo, Miguel Angel Chiurillo-
dc.typeArtigopt_BR
dc.titleGenome editing by crispr/cas9: a game change in the genetic manipulation of protistspt_BR
dc.contributor.authorLander, Noelia-
dc.contributor.authorChiurillo, Miguel A.-
dc.contributor.authorDocampo, Roberto-
dc.subjectGenomaspt_BR
dc.subject.otherlanguageGenomespt_BR
dc.description.abstractGenome editing by CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR‐associated gene 9) system has been transformative in biology. Originally discovered as an adaptive prokaryotic immune system, CRISPR/Cas9 has been repurposed for genome editing in a broad range of model organisms, from yeast to mammalian cells. Protist parasites are unicellular organisms producing important human diseases that affect millions of people around the world. For many of these diseases, such as malaria, Chagas disease, leishmaniasis and cryptosporidiosis, there are no effective treatments or vaccines available. The recent adaptation of the CRISPR/Cas9 technology to several protist models will be playing a key role in the functional study of their proteins, in the characterization of their metabolic pathways, and in the understanding of their biology, and will facilitate the search for new chemotherapeutic targets. In this work we review recent studies where the CRISPR/Cas9 system was adapted to protist parasites, particularly to Apicomplexans and trypanosomatids, emphasizing the different molecular strategies used for genome editing of each organism, as well as their advantages. We also discuss the potential usefulness of this technology in the green alga Chlamydomonas reinhardtiipt_BR
dc.relation.ispartofThe journal of eukaryotic microbiologypt_BR
dc.relation.ispartofabbreviationJ. eukaryot. microbiol.pt_BR
dc.publisher.cityHoboken, NJpt_BR
dc.publisher.countryEstados Unidospt_BR
dc.publisherWileypt_BR
dc.date.issued2016-
dc.date.monthofcirculationSept./Oct.pt_BR
dc.language.isoengpt_BR
dc.description.volume63pt_BR
dc.description.issuenumber5pt_BR
dc.description.firstpage679pt_BR
dc.description.lastpage690pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn1066-5234pt_BR
dc.identifier.eissn1550-7408pt_BR
dc.identifier.doi10.1111/jeu.12338pt_BR
dc.identifier.urlhttps://onlinelibrary.wiley.com/doi/full/10.1111/jeu.12338pt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber2013/50624-0; 2014/08995-4; 2014/13148-9pt_BR
dc.date.available2021-02-05T17:43:06Z-
dc.date.accessioned2021-02-05T17:43:06Z-
dc.description.provenanceSubmitted by Thais de Brito Barroso (tbrito@unicamp.br) on 2021-02-05T17:43:06Z No. of bitstreams: 0en
dc.description.provenanceMade available in DSpace on 2021-02-05T17:43:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2016en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/355295-
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.subject.keywordApicomplexapt_BR
dc.subject.keywordChlamydomonaspt_BR
dc.subject.keywordTrypanosomespt_BR
dc.identifier.source000382492500015pt_BR
dc.creator.orcid0000-0003-2178-381Xpt_BR
dc.creator.orcidSem informaçãopt_BR
dc.type.formArtigopt_BR
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