Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/355290
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampGonçalves, Frances Lilian Lanhellas-
dc.contributor.authorunicampBueno, Márcia Pereira-
dc.typeArtigopt_BR
dc.titleTreatment of bowel in experimental gastroschisis with a nitric oxide donorpt_BR
dc.contributor.authorGoncalves, Frances L. L.-
dc.contributor.authorBueno, Marcia P.-
dc.contributor.authorSchmidt, Augusto F.-
dc.contributor.authorFigueira, Rebeca L.-
dc.contributor.authorSbragia, Lourenco-
dc.subjectCirurgia experimentalpt_BR
dc.subjectGastrosquisept_BR
dc.subjectS-nitrosoglutationapt_BR
dc.subjectÓxido nítricopt_BR
dc.subject.otherlanguageSurgery, experimentalpt_BR
dc.subject.otherlanguageGastroschisispt_BR
dc.subject.otherlanguageS-nitrosoglutathionept_BR
dc.subject.otherlanguageNitric oxidept_BR
dc.description.abstractTo reduce the harmful effect of bowel exposure to amniotic fluid in gastroschisis, we used the nitric oxide (NO) donor S-nitrosoglutathione (GSNO) in an animal model of gastroschisis and assessed the ideal concentration for treatment of changes in bowel. Gastroschisis was surgically induced in rat fetuses on day 18.5 of gestation. The fetuses were divided into 5 groups (n = 12 animals/group): control (C), gastroschisis (G), gastroschisis + GSNO 5 mu mol/L (GNO1), gastroschisis + GSNO 0.5 mu mol/L (GNO2), and gastroschisis + GSNO 0.05 mu mol/L (GNO3). On day 21.5 of gestation, fetuses were collected by cesarean delivery. Body and intestinal weight were measured and the bowels were either fixed for histometric and immunohistochemical study or frozen for Western blotting. We analyzed bowel morphometry on histological sections and expression of the NO synthase (NOS) enzymes by Western blotting and immunohistochemistry. Data were analyzed by analysis of variance or Kruskal-Wallis test when appropriate. Morphological and histometric measurements of weight, diameter, and thickness of the layers of the intestinal wall decreased with GSNO treatment, especially in the GNO3 group, when compared with the G group (P < .05). The expression of neuronal NOS, endothelial NOS, and inducible NOS decreased mainly in GNO3 group compared to the G group (P < .05), with no difference compared to C group (P > .05). Fetal treatment with 0.05 mmol/L GSNO resulted in significant improvement of bowel morphology in gastroschisispt_BR
dc.relation.ispartofAmerican journal of obstetrics and gynecologypt_BR
dc.publisher.cityPhiladelphia, PApt_BR
dc.publisher.countryEstados Unidospt_BR
dc.publisherMosbypt_BR
dc.date.issued2015-
dc.date.monthofcirculationMar.pt_BR
dc.language.isoengpt_BR
dc.description.volume212pt_BR
dc.description.issuenumber3pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0002-9378pt_BR
dc.identifier.eissn1097-6868pt_BR
dc.identifier.doi10.1016/j.ajog.2014.09.025pt_BR
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0002937814009673pt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber08/51487-9; 11/00794-1; 08/52772-9; 11/12587-0pt_BR
dc.date.available2021-02-05T15:02:44Z-
dc.date.accessioned2021-02-05T15:02:44Z-
dc.description.provenanceSubmitted by Cintia Oliveira de Moura (cintiaom@unicamp.br) on 2021-02-05T15:02:44Z No. of bitstreams: 0en
dc.description.provenanceMade available in DSpace on 2021-02-05T15:02:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2015en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/355290-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.identifier.source000350377500038pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcidsem informaçãopt_BR
dc.type.formArtigopt_BR
dc.identifier.articleid383.e1pt_BR
dc.description.sponsorNoteHigher Education Consortia Program, Brazilian Ministry of Educationpt_BR
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