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Type: Artigo
Title: Prostate-specific RNA aptamer: promising nucleic acid antibody-like cancer detection
Author: Marangoni, Karina
Neves, Adriana F.
Rocha, Rafael M.
Faria, Paulo R.
Alves, Patrícia T.
Souza, Aline G.
Fujimura, Patrícia T.
Santos, Fabiana A. A.
Araújo, Thaise G.
Ward, Laura S.
Goulart, Luiz R.
Abstract: We described the selection of a novel nucleic acid antibody-like prostate cancer (PCa) that specifically binds to the single-stranded DNA molecule from a 277-nt fragment that may have been partially paired and bound to the PCA3 RNA conformational structure. PCA3-277 aptamer ligands were obtained and the best binding molecule, named CG3, was synthesized for validation. Aiming to prove its diagnostic utility, we used an apta-qPCR assay with CG3-aptamer conjugated to magnetic beads to capture PCA3 transcripts, which were amplified 97-fold and 7-fold higher than conventional qPCR in blood and tissue, respectively. Histopathologic analysis of 161 prostate biopsies arranged in a TMA and marked with biotin-labeled CG3-aptamer showed moderate staining in both cytoplasm and nucleus of PCa samples; in contrast, benign prostatic hyperplasia (BPH) samples presented strong nuclear staining (78% of the cases). No staining was observed in stromal cells. In addition, using an apta-qPCR, we demonstrated that CG3-aptamer specifically recognizes the conformational PCA3-277 molecule and at least three other transcript variants, indicating that long non-coding RNA (lncRNA) is processed after transcription. We suggest that CG3-aptamer may be a useful PCa diagnostic tool. In addition, this molecule may be used in drug design and drug delivery for PCa therapy
Subject: RNA
Country: Reino Unido
Editor: Springer Nature
Rights: Aberto
Identifier DOI: 10.1038/srep12090
Date Issue: 2015
Appears in Collections:FCM - Artigos e Outros Documentos

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