Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/355231
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampGoulart, Thais Marchi-
dc.typeArtigopt_BR
dc.titleMolecular detection of the blood meal source of sand flies (diptera: Psychodidae) in a transmission area of american cutaneous leishmaniasis, Parana state, Brazilpt_BR
dc.contributor.authorBaum, Mauricio-
dc.contributor.authorde Castro, Edilene Alcantara-
dc.contributor.authorPinto, Mara Cristina-
dc.contributor.authorGoulart, Thais Marchi-
dc.contributor.authorBaura, Walter-
dc.contributor.authorKlisiowicz, Debora do Rocio-
dc.contributor.authorVieira da Costa-Ribeiro, Magda Clara-
dc.subjectLeishmaniose tegumentar americanapt_BR
dc.subject.otherlanguageLeishmaniasis, Cutaneouspt_BR
dc.description.abstractThe feeding behavior of sand flies provides valuable information about the vector/host interactions and elucidates the epidemiological patterns of American cutaneous leishmaniasis (ACL) transmission. The aim of this study was to identify the blood meal sources of sand flies in endemic areas of leishmaniasis in Parana State through polymerase chain reaction (PCR) amplification of a prepronociceptin (PNOC) gene fragment and its subsequent DNA sequencing. Moreover, molecular assays were conducted to evaluate the sensitivity and reproducibility of the PNOC gene amplification. Besides that, a time-course digestion test of the blood using sand flies that fed artificially on BALB/c mice was performed. Of 1263 female sand flies collected in the field, 93 (3.6%) specimens were engorged and 27 allowed efficient amplification of the PNOC gene. These flies had fed on equine (Equus caballus), porcine (Sus scrofa) and canine (Canis lupus familiaris) species. The results also showed that the identification of the blood meal sources of the sand flies using the molecular method was directly linked to the level of digestion of the blood (time-course) and not to the amount of blood that had been ingested or to the presence of inhibitors in the bloodpt_BR
dc.relation.ispartofActa tropicapt_BR
dc.relation.ispartofabbreviationActa troppt_BR
dc.publisher.cityAmsterdampt_BR
dc.publisher.countryPaíses Baixospt_BR
dc.publisherElsevierpt_BR
dc.date.issued2015-
dc.date.monthofcirculationMar.pt_BR
dc.language.isoengpt_BR
dc.description.volume143pt_BR
dc.description.firstpage8pt_BR
dc.description.lastpage12pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0001-706Xpt_BR
dc.identifier.eissn1873-6254pt_BR
dc.identifier.doi10.1016/j.actatropica.2014.11.006pt_BR
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0001706X14003593pt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.date.available2021-02-03T21:46:50Z-
dc.date.accessioned2021-02-03T21:46:50Z-
dc.description.provenanceSubmitted by Cintia Oliveira de Moura (cintiaom@unicamp.br) on 2021-02-03T21:46:50Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-05-25T14:38:09Z : No. of bitstreams: 1 000350526800002.pdf: 358566 bytes, checksum: 8cf3b0d77e8373d751ffd36730ea8a82 (MD5)en
dc.description.provenanceMade available in DSpace on 2021-02-03T21:46:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2015en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/355231-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.identifier.source000350526800002pt_BR
dc.creator.orcid0000-0002-3271-365Xpt_BR
dc.type.formArtigopt_BR
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