Please use this identifier to cite or link to this item:
http://repositorio.unicamp.br/jspui/handle/REPOSIP/355166
Type: | Artigo |
Title: | 3D scaffolds to model the hematopoietic stem cell niche: applications and perspectives |
Author: | Congrains, Ada Bianco, Juares Rosa, Renata G. Mancuso, Rubia I. Saad, Sara T. O. |
Abstract: | Hematopoietic stem cells (HSC) are responsible for the production of blood and immune cells during life. HSC fate decisions are dependent on signals from specialized microenvironments in the bone marrow, termed niches. The HSC niche is a tridimensional environment that comprises cellular, chemical, and physical elements. Introductorily, we will revise the current knowledge of some relevant elements of the niche. Despite the importance of the niche in HSC function, most experimental approaches to study human HSCs use bidimensional models. Probably, this contributes to the failure in translating many in vitro findings into a clinical setting. Recreating the complexity of the bone marrow microenvironment in vitro would provide a powerful tool to achieve in vitro production of HSCs for transplantation, develop more effective therapies for hematologic malignancies and provide deeper insight into the HSC niche. We previously demonstrated that an optimized decellularization method can preserve with striking detail the ECM architecture of the bone marrow niche and support HSC culture. We will discuss the potential of this decellularized scaffold as HSC niche model. Besides decellularized scaffolds, several other methods have been reported to mimic some characteristics of the HSC niche. In this review, we will examine these models and their applications, advantages, and limitations |
Subject: | Medula óssea |
Country: | Suiça |
Editor: | MDPI |
Rights: | Aberto |
Identifier DOI: | 10.3390/ma14030569 |
Address: | https://www.mdpi.com/1996-1944/14/3/569 |
Date Issue: | 2021 |
Appears in Collections: | HEMO - Artigos e Outros Documentos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
103390ma14030569.pdf | 22.98 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.