Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/354987
Type: Artigo
Title: A novel human leiomyoma tissue derived matrix for cell culture studies
Author: Salo, Tuula
Sutinen, Meeri
Hoque Apu, Ehsanul
Sundquist, Elias
Cervigne, Nilva K.
Oliveira, Carine Ervolino de
Akram, Saad Ullah
Ohlmeier, Steffen
Suomi, Fumi
Eklund, Lauri
Juusela, Pirjo
Åström, Pirjo
Bitu, Carolina Cavalcante
Santala, Markku
Savolainen, Kalle
Korvala, Johanna
Paes Leme, Adriana Franco
Coletta, Ricardo D.
Abstract: The composition of the matrix molecules is important in in vitro cell culture experiments of e.g. human cancer invasion and vessel formation. Currently, the mouse Engelbreth-Holm-Swarm (EHS) sarcoma -derived products, such as Matrigel®, are the most commonly used tumor microenvironment (TME) mimicking matrices for experimental studies. However, since Matrigel® is non-human in origin, its molecular composition does not accurately simulate human TME. We have previously described a solid 3D organotypic myoma disc invasion assay, which is derived from human uterus benign leiomyoma tumor. Here, we describe the preparation and analyses of a processed, gelatinous leiomyoma matrix, named Myogel. A total protein extract, Myogel, was formulated from myoma. The protein contents of Myogel were characterized and its composition and properties compared with a commercial mouse Matrigel®. Myogel was tested and compared to Matrigel® in human cell adhesion, migration, invasion, colony formation, spheroid culture and vessel formation experiments, as well as in a 3D hanging drop video image analysis. We demonstrated that only 34 % of Myogel’s molecular content was similar to Matrigel®. All test results showed that Myogel was comparable with Matrigel®, and when mixed with low-melting agarose (Myogel-LMA) it was superior to Matrigel® in in vitro Transwell® invasion and capillary formation assays. In conclusion, we have developed a novel Myogel TME matrix, which is recommended for in vitro human cell culture experiments since it closely mimics the human tumor microenvironment of solid cancers
Subject: Leiomioma
Country: Reino Unido
Editor: Springer Nature
Rights: Aberto
Identifier DOI: 10.1186/s12885-015-1944-z
Address: https://bmccancer.biomedcentral.com/articles/10.1186/s12885-015-1944-z
Date Issue: 2015
Appears in Collections:FOP - Artigos e Outros Documentos

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