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http://repositorio.unicamp.br/jspui/handle/REPOSIP/354923
Type: | Artigo |
Title: | A simple protocol to determine lignin S/G ratio in plants by UHPLC-MS |
Author: | Mokochinski, Joao Benhur Bataglion, Giovana Anceski Kiyota, Eduardo Souza, Letícia Marrone de Mazzafera, Paulo Sawaya, Alexandra Christine Helena Frankland |
Abstract: | A simple extraction protocol and an ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) method for the determination of the syringyl/guaiacyl (S/G) ratio in lignin is reported herein. The method was entirely developed using stems of three Eucalyptus species, which were hydrolyzed with NaOH and partitioned with ethyl ether; vanillin (from the G monomer) and syringaldehyde (from S monomer) were quantified. The S/G ratios obtained were comparable to those usually reported for eucalyptus. The data for one of the eucalyptus species were compared with those obtained with a widely accepted method using thioacidolysis and gas chromatography-mass spectrometry (GC-MS). The method was also applied to sugarcane and showed to be reliable. The yield of the NaOH hydrolysis of the monolignols ranged from 89.94 to 95.69 %, with more than 77.12 % of recuperation in the liquid-liquid extraction. The whole analytical procedure was validated, achieving results with less than 4.38 % of variation. The lowest LOD and LOQ were 0.01 and 0.05 μg/mL, respectively. In addition, the method combines reliability and a fast and direct quantification |
Subject: | Lignina |
Country: | Alemanha |
Editor: | Springer |
Rights: | Fechado |
Identifier DOI: | 10.1007/s00216-015-8886-9 |
Address: | https://link.springer.com/article/10.1007/s00216-015-8886-9 |
Date Issue: | 2015 |
Appears in Collections: | IQ - Artigos e Outros Documentos IB - Artigos e Outros Documentos |
Files in This Item:
File | Description | Size | Format | |
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000360412100030.pdf | 654.55 kB | Adobe PDF | View/Open |
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