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Type: Artigo
Title: A simple protocol to determine lignin S/G ratio in plants by UHPLC-MS
Author: Mokochinski, Joao Benhur
Bataglion, Giovana Anceski
Kiyota, Eduardo
Souza, Letícia Marrone de
Mazzafera, Paulo
Sawaya, Alexandra Christine Helena Frankland
Abstract: A simple extraction protocol and an ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) method for the determination of the syringyl/guaiacyl (S/G) ratio in lignin is reported herein. The method was entirely developed using stems of three Eucalyptus species, which were hydrolyzed with NaOH and partitioned with ethyl ether; vanillin (from the G monomer) and syringaldehyde (from S monomer) were quantified. The S/G ratios obtained were comparable to those usually reported for eucalyptus. The data for one of the eucalyptus species were compared with those obtained with a widely accepted method using thioacidolysis and gas chromatography-mass spectrometry (GC-MS). The method was also applied to sugarcane and showed to be reliable. The yield of the NaOH hydrolysis of the monolignols ranged from 89.94 to 95.69 %, with more than 77.12 % of recuperation in the liquid-liquid extraction. The whole analytical procedure was validated, achieving results with less than 4.38 % of variation. The lowest LOD and LOQ were 0.01 and 0.05 μg/mL, respectively. In addition, the method combines reliability and a fast and direct quantification
Subject: Lignina
Country: Alemanha
Editor: Springer
Rights: Fechado
Identifier DOI: 10.1007/s00216-015-8886-9
Date Issue: 2015
Appears in Collections:IQ - Artigos e Outros Documentos
IB - Artigos e Outros Documentos

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