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Type: Artigo
Title: Topiramate effects lipolysis in 3T3-L1 adipocytes
Author: Martins, Gabriela Poltronieri Campagnaro
Souza, Camila Oliveira
Marques, Scherolin de Oliveira
Luciano, Thais Fernandes
Pieri, Bruno Luiz da Silva
Rosa, José César
Silva, Adelino Sanchez Ramos da
Pauli, José Rodrigo
Cintra, Dennys Esper
Ropelle, Eduardo Rochete
Rodrigues, Bruno
Lira, Fabio Santos de
Souza, Claudio Teodoro de
Abstract: Studies have shown that topiramate (TPM)-induced weight loss can be dependent on the central nervous system (CNS). However, the direct action of TPM on adipose tissue has not been tested previously. Thus, the present study aimed to examine whether TPM modulates lipolysis in 3T3‑L1. The 3T3‑L1 cells were incubated in 50 µM TPM for 30 min. The β‑adrenergic stimulator, isoproterenol, was used as a positive control. The release of lactate dehydrogenase, non‑esterified fatty acid, glycerol and incorporation of 14C‑palmitate to lipid were analyzed. The phosphorylation of protein kinase A (PKA), hormone‑sensitive lipase (HSL), adipocyte triglyceride lipase (ATGL) and perilipin A, as well as the protein levels of comparative genetic identification 58 (CGI‑58) were assessed. The levels of glycerol and non‑esterified fatty acid increased markedly when the cells were treated with TPM. The TPM effects were similar to the isoproterenol positive control. Additionally, TPM reduced lipogenesis. These results were observed without any change in cell viability. Finally, the phosphorylation of PKA, HSL, ATGL and perilipin A, as well as the protein levels of CGI‑58 were increased compared to the control cells. These results were similar to those observed in the cells treated with isoproterenol. The present results show that TPM increased the phosphorylation of pivotal lipolytic enzymes, which induced lipolysis in 3T3‑L1 adipocytes, suggesting that this drug may act directly in the adipose tissue independent from its effect on the CNS
Subject: Obesidade
Country: Grécia
Editor: Spandidos
Rights: Fechado
Identifier DOI: 10.3892/br.2015.514
Date Issue: 2015
Appears in Collections:FCA - Artigos e Outros Documentos

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